MABE939

Anti-phospho Histone H3 (Ser10), clone 6G8B7 Antibody

clone 6G8B7, 1 mg/mL, from rat

• Synonim(y): H3S10P, Histone H3 (phospho Ser10), H3 histone, family 3A, H3.3A, H3 histone, family 3B, H3.3B
• Kod UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• klon: 6G8B7, monoclonal
• application: ELISA; ICC; WB
• reaktywność gatunkowa: human
• metody: ELISA: suitable; immunocytochemistry: suitable; western blot: suitable
• citations: 4

Właściwości

• pochodzenie biologiczne: rat
• Poziom jakości: 100
• forma przeciwciała: purified antibody
• rodzaj przeciwciała: primary antibodies
• klon: 6G8B7, monoclonal
• reaktywność gatunkowa: human
• stężenie: 1 mg/mL
• metody: ELISA: suitable; immunocytochemistry: suitable; western blot: suitable
• izotyp: IgG2aκ
• numer dostępu NCBI: NP_002098
• numer dostępu UniProt: P84243
• Warunki transportu: wet ice
• docelowa modyfikacja potranslacyjna: phosphorylation (pSer10)
• informacje o genach: human ... H3C1(8350)

Opis

Opis ogólny

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Histone H3 variants (H3.1, H3.2 and H3.3) have been implicated in the epigenetic memory of cellular state. Genome-wide patterns of H3.3 are dependent on amino acid sequence and change with cellular differentiation at developmentally regulated loci.

Immunogen

KLH-conjugated linear peptide corresponding to human Histone H3 (Ser10).

Zastosowanie

Immunocytochemistry Analysis: A 1:5000 dilution from a representative lot detected Histone H3 (Ser10) in HeLa cells (Prof. Taro Tachibana, Osaka City University).
ELISA Analysis: A representative lot detected Histone H3 (Ser10) using differently phosphorylated and unphosphorylated peptides unmodified H3, H3 S10ph, and H3 S28ph (Prof. Taro Tachibana, Osaka City University).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Nuclear Receptors

This Anti-phospho Histone H3 (Ser10), clone 6G8B7 Antibody is validated for use in Western Blotting and Immunocytochemistry and ELISA for the detection of phospho Histone H3 (Ser10).

Jakość

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Histone H3 (Ser10) in 10 µg of nocodazole treated HeLa cell lysates.

Opis wartości docelowych

~17 kDa observed

Powiązanie

Replaces: 04-1093

Postać fizyczna

Format: Purified

Protein G Purified

Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Przechowywanie i stabilność

Stable for 1 year at 2-8°C from date of receipt.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
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