MABE644

Anti-Cre Recombinase Antibody, clone 5B4.1

clone 5B4.1, from mouse

• Synonim(y): Recombinase cre
• Kod UNSPSC: 12352203
• eCl@ss: 32160702

Właściwości

• pochodzenie biologiczne: mouse
• Poziom jakości: 100
• forma przeciwciała: purified immunoglobulin
• rodzaj przeciwciała: primary antibodies
• klon: 5B4.1, monoclonal
• reaktywność gatunkowa: virus
• reaktywność gatunkowa (przewidywana na podstawie homologii): all
• metody: western blot: suitable
• izotyp: IgG1κ
• numer dostępu NCBI: YP_006472
• numer dostępu UniProt: P06956
• Warunki transportu: ambient
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: Escherichia coli ... Cre(2777477)

Opis

Opis ogólny

Recombinase cre (UniProt P06956) is encoded by the cre gene (Gene ID 2777477) of bacteriophage P1. Cre (Causes recombination with Cyclization recombinase) is a 38 kDa DNA recombinase derived from the P1 bacteriophage. It is highly specific for a 34-bp DNA sequence motif called loxP (locus of X-over P1) found in P1 DNA. The loxP site is composed of a variable asymmetric 8-bp sequence in between two sets of 13-bp palindromic sequences (ATAACTTCGTATA-NNNTANNN-TATACGAAGTTAT). Two cre recombinase proteins, each binds to one of the two 13-bp palindromic sequences at a loxP site, form a dimer. This dimer then binds to a second dimer on another loxP site to form a tetramer. The two sites joined by the tetramer are parallel in orientation and the double stranded DNA is cut at both loxP sites. The strands are then rejoined with DNA ligase. Because the Cre gene and loxP sites are not native to most species, they are often introduced into target cells or tissues and employed as a means to precisely control the expression of genes both in cultured cells in vitro and in animals vivo.

Specyficzność

Clone 5B4.1 detects an epitope outside the N-terminal region.

Immunogen

GST-tagged recombinant Cre lacking the N-terminal region sequence.

Zastosowanie

Anti-Cre Recombinase Antibody, clone 5B4.1, Cat. No. MABE644, is a highly specific mouse monoclonal antibody that targets Cre recombinase and has been tested in Western Blotting.

Research Category
Epigenetics & Nuclear Function

Jakość

Evaluated by Western Blotting in recombinant Cre.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected 250 ng of recombinant Cre (with added N- and C-terminal tag sequences).

Opis wartości docelowych

38.54 kDa calculated.

Powiązanie

Replaces: MABE3120

Postać fizyczna

Format: Purified

Protein G purified.

Purified mouse IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Przechowywanie i stabilność

Stable for 1 year at 2-8°C from date of receipt.

Inne uwagi

Concentration: Please refer to lot specific datasheet.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable