MAB3120
Anti-Cre Recombinase Antibody, clone 2D8
ascites fluid, clone 2D8, Chemicon®
Synonim(y):
Anti-Cre Antibody
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About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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pochodzenie biologiczne
mouse
Poziom jakości
forma przeciwciała
ascites fluid
rodzaj przeciwciała
primary antibodies
klon
2D8, monoclonal
reaktywność gatunkowa (przewidywana na podstawie homologii)
all
producent / nazwa handlowa
Chemicon®
metody
ELISA: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable
izotyp
IgG1κ
numer dostępu UniProt
Warunki transportu
dry ice
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
Escherichia coli ... Cre(2777477)
Opis ogólny
Cre-recombinase is a 38 kDa DNA recombinase derived from the P1 bacteriophage. It is highly specific for a 34 bp DNA sequence (loxP) found in P1 DNA. It catalyzes site-specific recombination between two 34-base-pair LOXP sites. Its role is to maintain the phage genome as a monomeric unit-copy plasmid in the lysogenic state. It is homotetramer when bound to DNA and belongs to the "phage" integrase family. Anti-Cre Recombinase Antibody can be used to determine the success of gene manipulations in transgenic animals.
Specyficzność
Cre recombinase. The epitope is located between aa 77 and 343.
Immunogen
Cre recombinase (aa 77 to 343) fusion protein
Zastosowanie
Anti-Cre Recombinase Antibody, clone 2D8 is a well characterized monoclonal antibody. Proven to reliably detect Cre Recombinase, this mAb is validated for use in ELISA, IC, IF, IH & WB and is backed by multiple publications.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair
Cell Cycle, DNA Replication & Repair
Western Blot (natural and recombinant protein):
A 1:500-1:5,000 dilution was used in a previous lot. Natural Cre recombinase from Bacteriophage P1 migrates at 38.5 kDa by SDS-PAGE.
Immunofluorescence:
A previous lot of this antibody was used on IF.
Immunohistochemistry:
A 1:500-1:5,000 dilution of a previous lot was used in IH.
ELISA:
A 1:500-1:5,000 dilution of a previous lot was used in ELISA.
Optimal working dilutions must be determined by the end user.
A 1:500-1:5,000 dilution was used in a previous lot. Natural Cre recombinase from Bacteriophage P1 migrates at 38.5 kDa by SDS-PAGE.
Immunofluorescence:
A previous lot of this antibody was used on IF.
Immunohistochemistry:
A 1:500-1:5,000 dilution of a previous lot was used in IH.
ELISA:
A 1:500-1:5,000 dilution of a previous lot was used in ELISA.
Optimal working dilutions must be determined by the end user.
Opis wartości docelowych
38 kDa
Postać fizyczna
Ascites fluid. Contains no preservative
Unpurified
Przechowywanie i stabilność
Stable for 1 year at -20ºC from date of receipt.
Komentarz do analizy
Control
HeLa Nuclear Extract
HeLa Nuclear Extract
Informacje prawne
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania
10 - Combustible liquids
Klasa zagrożenia wodnego (WGK)
WGK 1
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
Masz już ten produkt?
Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Murine cell lines derived from Pten null prostate cancer show the critical role of PTEN in hormone refractory prostate cancer development.
Jiao, J; Wang, S; Qiao, R; Vivanco, I; Watson, PA; Sawyers, CL; Wu, H
Cancer Research null
Hong-Xiang Liu et al.
Developmental biology, 368(2), 294-303 (2012-06-05)
The epithelium of mammalian tongue hosts most of the taste buds that transduce gustatory stimuli into neural signals. In the field of taste biology, taste bud cells have been described as arising from "local epithelium", in distinction from many other
Generation of a transgenic mouse in which Cre recombinase is expressed under control of the type II collagen promoter and doxycycline administration.
Judy Grover, Peter J Roughley
Matrix Biology null
Axon position within the corpus callosum determines contralateral cortical projection.
Zhou, J; Wen, Y; She, L; Sui, YN; Liu, L; Richards, LJ; Poo, MM
Proceedings of the National Academy of Sciences of the USA null
Quality control of astrocyte-directed Cre transgenic mice: the benefits of a direct link between loss of gene expression and reporter activation.
Robert Pascal Requardt, Lech Kaczmarczyk, Pavel Dublin, Anke Wallraff-Beck et al.
Glia null
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