MABE343-AF647
Anti-Puromycin, clone 12D10, Alexa Fluor™ 647 Conjugate Antibody
clone 12D10, 0.5 mg/mL, from mouse
Synonim(y):
Alexa Fluor 647 Anti-Puromycin, Clone 12D10 Anti-Puromycin, Puromycin Detection Antibody
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About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Polecane produkty
pochodzenie biologiczne
mouse
Poziom jakości
białko sprzężone
ALEXA FLUOR™ 647
forma przeciwciała
purified antibody
rodzaj przeciwciała
primary antibodies
klon
12D10, monoclonal
reaktywność gatunkowa
human
stężenie
0.5 mg/mL
metody
immunocytochemistry: suitable
Warunki transportu
wet ice
docelowa modyfikacja potranslacyjna
unmodified
Opis ogólny
Puromycin is an aminonucleoside antibiotic, derived from the Streptomyces alboniger bacterium, that functions as a protein synthesis inhibitor that blocks translation through premature chain termination in the ribosome. Monoclonal antibodies to puromycin may be used with standard immunochemical methods to directly monitor translation, a method known as surface sensing of translation (SUnSET). Part of the molecule resembles the 3′ end of the aminoacylated tRNA, making it useful for protein translation analysis. Puromycin induces DNA fragmentation in thymocytes and in human HL-60 leukemia cells.
This Anti-Puromycin, clone 12D10 is validated for use in Immunocytochemistry for the detection of Puromycin.
Specyficzność
Predicted to react with cells from all species when pre-incubated with puromycin.
Jakość
Evaluated by Immunocytochemistry Analysis untreated and puromycin treated HeLa cells.
Immunocytochemistry Analysis: A 1:2,500 dilution of this antibody detected puromycin-incorporated neosynthesized proteins in puromycin treated HeLa cells.
Alexa Fluor™ is a registered trademark of Life Technologies.
Immunocytochemistry Analysis: A 1:2,500 dilution of this antibody detected puromycin-incorporated neosynthesized proteins in puromycin treated HeLa cells.
Alexa Fluor™ is a registered trademark of Life Technologies.
Opis wartości docelowych
Refer to Cat. No. MABE343 for observed molecular weight information.
Informacje prawne
ALEXA FLUOR is a trademark of Life Technologies
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Kod klasy składowania
12 - Non Combustible Liquids
Klasa zagrożenia wodnego (WGK)
WGK 2
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
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