MABE325
Anti-p21WAF1 Antibody, clone EA10
clone EA10, from mouse
Synonim(y):
Cyclin-dependent kinase inhibitor 1, CDK-interacting protein 1, Melanoma differentiation-associated protein 6, MDA-6, p21
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About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Polecane produkty
pochodzenie biologiczne
mouse
Poziom jakości
forma przeciwciała
purified immunoglobulin
rodzaj przeciwciała
primary antibodies
klon
EA10, monoclonal
reaktywność gatunkowa
human
metody
flow cytometry: suitable
western blot: suitable
izotyp
IgG1κ
numer dostępu NCBI
numer dostępu UniProt
Warunki transportu
wet ice
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
human ... CDKN1A(1026)
Opis ogólny
The tumor suppressor p53 transcriptionally activates a number of genes including the WAF1/CIP1 gene in response to DNA damage. The ~21 kDa product of the WAF1 gene is found in a complex involving cyclins, CDKs, and PCNA in normal cells but not transformed cells and appears to be a universal inhibitor of CDK activity. One consequence of p21WAF1 binding to and inhibiting CDKs is the prevention of CDK-dependent phosphorylation and subsequent inactivation of the Rb protein which is essential for cell cycle progression. p21WAF1 is, therefore, a potent and reversible inhibitor of cell cycle progression at both the G1 and G2 checkpoints, presumably to allow sufficient time for DNA repair to be completed. Irreversible G1 or G2 arrest leads to apoptosis. While the role of p21WAF1 in apoptosis is less clear, it is known that p53-mediated apoptosis leads to increased WAF1 expression. Induction of p21WAF1 can occur by both p53-dependent and p53-independent mechanisms, in response to certain observed conditions. p21WAF1 has also been identified as a gene involved in cellular senescence, termed sdi1. Its overexpression was observed to inhibit cellular growth.
Immunogen
Recombinant protein corresponding to human p21WAF1.
Zastosowanie
Anti-p21WAF1 Antibody, clone EA10 is a Mouse Monoclonal Antibody for detection of p21WAF1 & has been validated in WB, FC.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair
Cell Cycle, DNA Replication & Repair
Western Blot Analysis: A representative lot from an independent laboratory detected p21WAF1 in non-irradiated and gamma irradiated CLL cells (Carter, A., et al. (2004). Br J Haematol. 127(4):425-428).
Immunohistochemistry Analysis: A representative lot from an independent laboratory detected p21WAF1 in normal human lower back tissue (el-Deiry, W. S., et al. (1995). Cancer Res. 55(13):2910-2919.).
Flow Cytometry Analysis: A representative lot from an independent laboratory detected p21WAF1 in non-irradiated and gamma irradiated CLL cells (Carter, A., et al. (2004). Br J Haematol. 127(4):425-428).
Immunohistochemistry Analysis: A representative lot from an independent laboratory detected p21WAF1 in normal human lower back tissue (el-Deiry, W. S., et al. (1995). Cancer Res. 55(13):2910-2919.).
Flow Cytometry Analysis: A representative lot from an independent laboratory detected p21WAF1 in non-irradiated and gamma irradiated CLL cells (Carter, A., et al. (2004). Br J Haematol. 127(4):425-428).
Jakość
Evaluated by Western Blot in HT-29 cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected p21WAF1 in 10 µg of HT-29 cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected p21WAF1 in 10 µg of HT-29 cell lysate.
Opis wartości docelowych
~21 kDa observed
Postać fizyczna
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Przechowywanie i stabilność
Stable for 1 year at 2-8°C from date of receipt.
Komentarz do analizy
Control
HT-29 cell lysate
HT-29 cell lysate
Inne uwagi
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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polecane
Kod klasy składowania
12 - Non Combustible Liquids
Klasa zagrożenia wodnego (WGK)
WGK 1
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Detection of p53 dysfunction by flow cytometry in chronic lymphocytic leukaemia.
Carter, A, et al.
British Journal of Haematology, 127, 425-428 (2004)
Yi-He Ling et al.
Molecular pharmacology, 72(2), 248-258 (2007-04-26)
Erlotinib, a small-molecule epidermal growth factor receptor (EGFR) tyrosine kinase inhibitor, has been shown to have potent antitumor effects against human non-small-cell lung cancer (NSCLC) cell growth; however, the mechanism of such an effect is not elucidated. Here, we demonstrate
P Giannakakou et al.
Oncogene, 20(29), 3806-3813 (2001-07-06)
Paclitaxel (PTX), a microtubule-active agent, blocks cell proliferation by inhibiting mitotic progression leading to mitotic and postmitotic arrest and cell death. Here we demonstrate for the first time that very low concentrations of PTX (3-6 nM) can completely inhibit cell
Juan M Roldan-Romero et al.
International journal of cancer, 146(5), 1435-1444 (2019-07-25)
The mammalian target of rapamycin (mTOR) pathway inhibitors are key drugs for the treatment of many tumor types, however, there are no predictive biomarkers in clinical use. Here, we performed a molecular and immunohistochemical characterization of key mTOR pathway components
W S el-Deiry et al.
Cancer research, 55(13), 2910-2919 (1995-07-01)
The p53-regulated gene product p21WAF1/CIP1 is the prototype of a family of small proteins that negatively regulate the cell cycle. To learn more about p21WAF1/CIP1 regulation in vivo, monoclonal antibodies were developed for immunohistochemistry. These revealed that p21WAF1/CIP1 expression followed
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