MABE251

Anti-5-hydroxymethylcytosine (5hmC) Antibody, clone HMC 31

clone HMC 31, from mouse

• Kod UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: mouse
• Poziom jakości: 100
• forma przeciwciała: purified immunoglobulin
• rodzaj przeciwciała: primary antibodies
• klon: HMC 31, monoclonal
• reaktywność gatunkowa (przewidywana na podstawie homologii): all
• metody: ELISA: suitable; dot blot: suitable; methylated DNA immunoprecipitation (MeDIP): suitable
• izotyp: IgG1κ
• Warunki transportu: wet ice
• docelowa modyfikacja potranslacyjna: unmodified

Opis

Opis ogólny

5-Hydroxymethylcytosine is a DNA pyrimidine nitrogen base formed from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of iron-dependent oxygenases. Data suggests that every mammilian cell contains 5-hydroxymethylcytosine, but the levels vary depending on the cell type; data also suggests that levels of 5-hydroxymethylcytosine increases with age. The highest levels are found in neuronal cells of the central nervous system and certain mammalian tissues such as mouse Purkinje and granule neurons. Although the exact function has not been fully elucidated, studies suggest that 5-hydroxymethlcytosine may regulate gene expression or initiate DNA demethylation.

Specyficzność

This antibody recognizes the 5-hydroxymethylcytosine of 5-Hydroxymethylcytosine DNA.

Immunogen

BSA-conjugated 5-hydroxymethylcytosine.

Epitope: 5-hydroxymethylcytosine

Zastosowanie

Detect 5-hydroxymethylcytosine (5hmC) with Anti-5-hydroxymethylcytosine (5hmC) Antibody, clone HMC 31 (Mouse Monoclonal Antibody), that has been shown to work in DB, MeDIP, ELISA.

ELISA Analysis: A representative lot detected 5-hydroxymethylcytosine in ELISA.

Methylated DNA Immunoprecipitation (MeDIP) Analysis: A representative lot immunoprecipitated 5-hydroxymethylcytosine in ChIP.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Chromatin Biology

Jakość

Evaluated by Dot Blot in unmodified and modified cytosine DNA.

Dot Blot Analysis: 0.1 µg/mL of this antibody detected 5-hydroxymethylcytosine in 10 ng and 50 ng of unmodified and modified cytosine DNA.

Opis wartości docelowych

This antibody detects 5-hydroxymethylcytosine.

Postać fizyczna

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Przechowywanie i stabilność

Stable for 1 year at 2-8°C from date of receipt.

Komentarz do analizy

Control
Unmodified and modified cytosine DNA.

Inne uwagi

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable