MABE146
Anti-5-methylcytosine Antibody, clone 33D3
clone 33D3, from mouse
Synonim(y):
methylcytosine, methylcytidine
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About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Polecane produkty
pochodzenie biologiczne
mouse
Poziom jakości
forma przeciwciała
purified immunoglobulin
rodzaj przeciwciała
primary antibodies
klon
33D3, monoclonal
reaktywność gatunkowa
human, mouse
metody
Southern blotting: suitable
dot blot: suitable
flow cytometry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable (paraffin)
methylated DNA immunoprecipitation (MeDIP): suitable
izotyp
IgG1λ
Warunki transportu
wet ice
docelowa modyfikacja potranslacyjna
unmodified
Opis ogólny
5-Methylcytosine is a modified base found in the DNA of higher eukaryotes. Methylation of DNA is known to be involved in the control of gene expression and is mediated by a class of enzymes known as DNA methyltransferases (DNMT’s). Appropriate cytosine methylation is essential for normal development and maintenance of mammalian cells. Alternations in DNA methylation patterns is often associated with cancers with the turmor DNA being hypomethylated compared to DNA from normal tissues.
Specyficzność
Predicted to react with multiple species.
This antibody detects 5-Methylcytosine, but not unmethylated cytosine.
Immunogen
Ovalbumin-conjugated 5-methylcytosine
Zastosowanie
IF
Habib, M., et al. (1999). Exp Cell Res. 249:46-53
IHC-P
Hernadez-Blazquez, F.J., et al. (2000). Gut. 47:689-693
Flow Cytometry
Reynaud, C.C., et al. (1992). Cancer Lett. 61(3):255-262
MeDIP
Keshet, I., et al. (2006). Nat Genet. 38(2):149-153
Habib, M., et al. (1999). Exp Cell Res. 249:46-53
IHC-P
Hernadez-Blazquez, F.J., et al. (2000). Gut. 47:689-693
Flow Cytometry
Reynaud, C.C., et al. (1992). Cancer Lett. 61(3):255-262
MeDIP
Keshet, I., et al. (2006). Nat Genet. 38(2):149-153
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology
Chromatin Biology
Use Anti-5-methylcytosine Antibody, clone 33D3 (mouse monoclonal antibody) validated in DB, IF, SB, IHC(P), Flow Cytometry, MeDIP to detect 5-methylcytosine also known as methylcytosine, methylcytidine.
Jakość
Evaluated by Dot Blot in methylated and unmethylated BRCA1.
Dot Blot Analysis: 1 µg/mL of this antibody detected methylcytosine in methylated and unmethylated BRCA1.
Dot Blot Analysis: 1 µg/mL of this antibody detected methylcytosine in methylated and unmethylated BRCA1.
Opis wartości docelowych
Detects methylated cytosine
Powiązanie
Replaces: MABE527
Postać fizyczna
Format: Purified
Protein A
Purified mouse monoclonal IgG1λ in buffer containing PBS and 0.05% sodium azide.
Przechowywanie i stabilność
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Komentarz do analizy
Control
Methylated and unmethylated BRCA1
Methylated and unmethylated BRCA1
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania
10 - Combustible liquids
Klasa zagrożenia wodnego (WGK)
WGK 2
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
Masz już ten produkt?
Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Evidence for an instructive mechanism of de novo methylation in cancer cells.
Keshet, Ilana, et al.
Nature Genetics, 38, 149-153 (2006)
Abnormal methylation pattern in constitutive and facultative (X inactive chromosome) heterochromatin of ICF patients.
Miniou, P, et al.
Human Molecular Genetics, 3, 2093-2102 (1994)
Methylation of genomes and genes at the invertebrate-vertebrate boundary.
Tweedie, S, et al.
Molecular and cellular biology, 17, 1469-1475 (1997)
Alain Cuna et al.
American journal of respiratory cell and molecular biology, 53(1), 60-73 (2014-11-12)
DNA methylation, a major epigenetic mechanism, may regulate coordinated expression of multiple genes at specific time points during alveolar septation in lung development. The objective of this study was to identify genes regulated by methylation during normal septation in mice
Polymerase chain reaction products containing 5-methyldeoxycytidine: a microplate immunoquantitation method.
Niveleau, A, et al.
Nucleic Acids Research, 22, 5508-5509 (1994)
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