MABE1877

Anti-EBNA-1 Antibody, clone 1EB14

• Kod UNSPSC: 12352203
• NACRES: NA.74

Właściwości

• pochodzenie biologiczne: mouse
• Poziom jakości: 200
• białko sprzężone: unconjugated
• forma przeciwciała: purified antibody
• rodzaj przeciwciała: primary antibodies
• klon: 1EB14, monoclonal
• masa cząsteczkowa: calculated mol wt 56.43 kDa; observed mol wt ~60 kDa
• oczyszczone przez: using protein G
• reaktywność gatunkowa: virus
• opakowanie: antibody small pack of 100 μg
• metody: ELISA: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• izotyp: IgG1κ
• sekwencja epitopowa: C-terminal half
• numer dostępu UniProt: P03211
• Warunki transportu: 2-8°C
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: vaccinia virus ... EBNA-1(3783774)

Opis

Opis ogólny

Epstein-Barr nuclear antigen 1 (UniProt: P03211; also known as EBNA-1, EBV nuclear antigen 1) is encoded by the EBNA1 (also known as BKRF1) gene (Gene ID: 3783709) in Epstein-Barr virus (EBV). EBV is a gamma-herpesvirus, a causative agent of infectious mononucleosis. EBNA1, a multi-functional protein, plays an important role in maintaining EBV in the host cell and has been implicated in host cell immortalization. It plays an essential role in replication and partitioning of viral genomic DNA during latent viral infection. During this phase, the circular double-stranded viral DNA undergoes replication once per cell cycle and is efficiently partitioned to the daughter cells. EBNA1 activates the initiation of viral DNA replication through binding to specific sites in the viral latent origin of replication, oriP. It is also reported to direct the segregation of viral episomes by mediating their attachment to host cell metaphase chromosomes and is known to activate the transcription of several viral latency genes. By counteracting the stabilization of host p53, it also reduces apoptosis and increases host cell survival. EBNA1 is also a risk factor in a variety of malignancies and is the only viral protein found in all EBV-related malignancies. Its C-terminal region contains a dimerization domain and a DNA binding domain (aa 459-607). Although this domain is essential for its replication function of EBNA1, it is not sufficient for this activity and the N-terminal half of EBNA1 is also required. (Ref.: Sun, X., et al. (2010). Proc. Natl. Acad. Sci. USA. 107(7); 3146-3151; Duellman, SJ., and Burgess, RR. (2009). PLoS ONE 4(2): e4614; Wu, H., et al. (2002). J. Virol. 76(5); 2480-2490).

Specyficzność

Clone 1EB14 is a mouse monoclonal antibody that detects Epstein-Barr nuclear antigen 1 (EBNA-1). It targets an epitope within 13 amino acids from the C-terminal half.

Immunogen

His-tagged recombinant Epstein-Barr nuclear antigen 1 expressed in E. coli.

Zastosowanie

Quality Control Testing

Evaluated by Western Blotting in Raji cell lysate.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected EBNA-1 in Raji cell lysate.

Tested Applications

Immunoprecipitation Analysis: A representative lot immunoprecipitated EBNA-1 in Immunoprecipitation application. (Duellman, S.J., et al. (2009). PLoS One. 4(2):e4614).

ELISA Analysis: A representative lot detected EBNA-1 in ELISA application. (Duellman, S.J., et al. (2009). PLoS One. 4(2):e4614).

Western Blotting Analysis: A representative lot detected EBNA-1 in Western Blotting application. (Duellman, S.J., et al. (2009). PLoS One. 4(2):e4614; Duellman, S. J., et al. (2006). Protein Expr Purif. 47(2):434--40).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-EBNA-1, clone 1EB14, Cat. No. MABE1877, is a mouse monoclonal antibody that detects Epstein-Barr nuclear antigen 1 and is tested in ELISA, Immunoprecipitation, and Western Blotting.

Postać fizyczna

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Przechowywanie i stabilność

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

Inne uwagi

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable