MAB5272
Anti-Neural Cell Adhesion Molecule L1 Antibody, clone 324
clone 324, Chemicon®, from rat
Synonim(y):
CD171, N-CAM L1
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About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Polecane produkty
pochodzenie biologiczne
rat
Poziom jakości
forma przeciwciała
purified antibody
rodzaj przeciwciała
primary antibodies
klon
324, monoclonal
reaktywność gatunkowa
mouse, rat
producent / nazwa handlowa
Chemicon®
metody
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
izotyp
IgG
numer dostępu NCBI
numer dostępu UniProt
Warunki transportu
wet ice
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
human ... L1CAM(3897) , VCAM1(7412)
Opis ogólny
Families of adhesion molecules which share common carbohydrate domains do exist, despite the structural and functional diversity of these glycoproteins. These include the Ca2+-independent neural adhesion molecules: N-CAM, myelin associated glycoprotein (MAG) and L1. L1 is involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, cerebellar granule cell migration, neurite outgrowth on Schwann cells and interactions among epithelial cells of intestinal crypts
Specyficzność
Monoclonal antibody against neural cell adhesion molecule L1 from rat-rat hybrid cells.
Immunogen
Rats were immunized with a glycoprotein fraction from cerebellum of 8-10 day old C57BL/6J mice.
Zastosowanie
This Anti-Neural Cell Adhesion Molecule L1 Antibody, clone 324 is validated for use in IC, IH, WB for the detection of Neural Cell Adhesion Molecule L1.
Western blotting:
20 μg/mL was used on a previous lot.
Immunocytochemistry:
5-10 μg/mL was used on a previous lot. Cells must be fixed with methanol for 10 minutes at -20°C in the middle log phase.
Immunohistochemistry: Clone 324 is sensitive to fixation. Fresh frozen, acetone or -20C methanol fixed tissues are recommended. Traditional 4% PFA typically does not work well. Other fixatives have not been tested.
Optimal working dilutions must be determined by end user.
20 μg/mL was used on a previous lot.
Immunocytochemistry:
5-10 μg/mL was used on a previous lot. Cells must be fixed with methanol for 10 minutes at -20°C in the middle log phase.
Immunohistochemistry: Clone 324 is sensitive to fixation. Fresh frozen, acetone or -20C methanol fixed tissues are recommended. Traditional 4% PFA typically does not work well. Other fixatives have not been tested.
Optimal working dilutions must be determined by end user.
Opis wartości docelowych
120-140 kDa
Postać fizyczna
Format: Purified
Rat monoclonal in buffer containing 0.02 M Phosphate buffer, 0.25 M NaCl, pH 7.6 with 0.1% sodium azide.
Inne uwagi
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informacje prawne
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Kod klasy składowania
10 - Combustible liquids
Klasa zagrożenia wodnego (WGK)
WGK 2
Certyfikaty analizy (CoA)
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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
L1 mono- and polyclonal antibodies modify cell migration in early postnatal mouse cerebellum.
Lindner, J, et al.
Nature, 305, 427-430 (1983)
Absence of layer-specific cadherin expression profiles in the neocortex of the reeler mutant mouse.
Hertel, N; Redies, C
Cerebral Cortex (1991)
Heparan sulfate sugar modifications mediate the functions of slits and other factors needed for mouse forebrain commissure development.
Conway, CD; Howe, KM; Nettleton, NK; Price, DJ; Mason, JO; Pratt, T
The Journal of Neuroscience null
Expression of ABCA2 protein in both non-myelin-forming and myelin-forming Schwann cells in the rodent peripheral nerve.
Takashi Saito, Katsuya Yamada, Yan Wang, Yukiko Tanaka, Kazuo Ohtomo, Kazuo Ishikawa, Nobuya Inagaki
Neuroscience Letters null
Neuron glia-related cell adhesion molecule (NrCAM) promotes topographic retinocollicular mapping.
Dai, J; Buhusi, M; Demyanenko, GP; Brennaman, LH; Hruska, M; Dalva, MB; Maness, PF
Testing null
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