MAB4444

Anti-Epithelial Cell Adhesion Molecule (EpCAM) Antibody, clone 8

clone mab 8, from mouse

• Synonim(y): Adenocarcinoma-associated antigen, CD326 antigen, Cell surface glycoprotein Trop-1, Epithelial cell surface antigen, Epithelial glycoprotein, KS 1/4 antigen, Major gastrointestinal tumor-associated protein GA733-2, antigen identified by monoclonal antibo
• Kod UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: mouse
• Poziom jakości: 100
• forma przeciwciała: purified immunoglobulin
• klon: mab 8, monoclonal
• reaktywność gatunkowa: human
• metody: flow cytometry: suitable; immunocytochemistry: suitable; western blot: suitable
• izotyp: IgG2aκ
• numer dostępu NCBI: NP_002345.2
• numer dostępu UniProt: P16422
• Warunki transportu: wet ice
• docelowa modyfikacja potranslacyjna: unmodified

Opis

Opis ogólny

Epithelial Call Adhesion Molecule (EpCAM, CD326) is a transmembrane glycoprotein of approximately 40 kDa. EpCAM was initially identified as a tumor associated marker having high expression in quickly proliferating epithelial tumors. As such, EpCAM has been of significant interest as a means for tumor diagnosis and therapy. EpCAM is also expressed in normal epithelial cells, but in comparatively lower levels. Preliminary studies limited EpCAM’s function to cell-cell adhesion; however more recent studies indicate it to be involved in diverse processes that include cell migration, cell signaling, differentiation and proliferation.

Specyficzność

This antibody recognizes epithelial cell adhesion molecule (EpCAM), specifically on undifferentiated human embryonic stem cells.

Immunogen

Epitope: Unknown

HES-3 Cells (Choo, et al. (2008) Stem Cells. Jun;26(6):1454-63)

Zastosowanie

Flow Cytometry Analysis: 0.5 µg from a previous lot detected Epithelial cell adhesion molecule in human embryonic stem cells.

Western Blot Analysis: A previous lot was used by an independent laboratory in WB. (Ng, V., et al., 2010)

Research Category
Stem Cell Research

Research Sub Category
Pluripotent & Early Differentiation

Adhesion (CAMs)

This Anti-Epithelial Cell Adhesion Molecule (EpCAM) Antibody, clone 8 is validated for use in FC, IC, WB for the detection of Epithelial Cell Adhesion Molecule (EpCAM).

Jakość

Evaluated by Immunocytochemistry in human embryonic stem cells.

Immunocytochemistry Analysis: 1-10 µg/mL from a previous lot detected epithelial cell adhesion molecule (EpCAM) in human embryonic stem cells.

Opis wartości docelowych

~ 40 kDa

Postać fizyczna

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Przechowywanie i stabilność

Stable for 1 year at 2-8°C from date of receipt.

Komentarz do analizy

Control
Human embryonic stem cells

Inne uwagi

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Ta strona może zawierać tekst przetłumaczony maszynowo.

Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable