MAB4197
Anti-Topoisomerase II Antibody, clone KiS1
clone KiS1, Chemicon®, from mouse
Synonim(y):
Ki-S1
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About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Polecane produkty
pochodzenie biologiczne
mouse
Poziom jakości
forma przeciwciała
purified immunoglobulin
klon
KiS1, monoclonal
reaktywność gatunkowa
human
producent / nazwa handlowa
Chemicon®
metody
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
izotyp
IgG2a
numer dostępu NCBI
numer dostępu UniProt
Warunki transportu
wet ice
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
human ... TOP2A(7153)
Opis ogólny
Topoisomerase II β (M.W 180 kDa) plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is present ubiquitously in normal cells and is upregulated in tumors and proliferating cells. Topoisomerase II β is also implicated in drug resistance of tumor cells.
Specyficzność
In Immunoprecipitation and Western blot experiments the Ki-S1 antibody recognizes a major protein of 170 kD which was identified as the a isoform of topoisomerase II (Boege et al., 1995; Kreipe et al., 1993). In addition, it has been shown that the Ki-S1 antibody recognizes a carboxyterminal a-isoenzyme specific epitope missing in topoisomerase IIb (Boege et al., 1995). In immunohistochemistry the Ki-S1 antibody shows strong nuclear staining only in proliferating cells. The epitope recognized by the antibody is also detectable in paraffin-embedded tissue sections (Kreipe et al., 1993). Accordingly, it has been shown that the expression of topoisomerase IIa is strongly restricted to proliferating cells (Tsutsui et al., 1993). The topoisomerase IIa antigen is preferentially expressed during G 1 , S, G 2 and M phase of the cell cycle, while resting, non-cycling cells (G 0 phase) lack topoisomerase IIa. In addition, constantly proliferating cells (e.g. cell lines) react positively to topoisomerase IIa during the entire cell-cycle. This specificity of Ki-S1 antibody for proliferating cells might make it a useful tool for determination of the proliferative fraction in solid tumors such as mammary carcinomas (Kreipe et al., 1993; Sampson et al., 1992; Camplejohn et al., 1993; Kreipe et al., 1993; Rasbridge et al., 1994) and gangliomas (Wolf et al., 1994).
Zastosowanie
Anti-Topoisomerase II Antibody, clone KiS1 is a Mouse Monoclonal Antibody for detection of Topoisomerase II also known as Ki-S1 & has been validated in FC, WB, ICC, IHC, IHC(P).
Western blot: 1-10μg/ml Immunoprecipitaion
Immunocytochemistry: 5-10 μg/ml Immunohistochemistry: 5-10 μg/ml
Flow cytometry
Optimal working dilutions must be determined by end user.
Immunocytochemistry: 5-10 μg/ml Immunohistochemistry: 5-10 μg/ml
Flow cytometry
Optimal working dilutions must be determined by end user.
Powiązanie
Replaces: MABE519
Postać fizyczna
Format: Purified
Przechowywanie i stabilność
Maintain at 2-8°C in undiluted aliquots for up to 6 months.Avoid repeat freeze/thaw cycles.
Inne uwagi
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informacje prawne
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Kod klasy składowania
12 - Non Combustible Liquids
Klasa zagrożenia wodnego (WGK)
WGK 2
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
Masz już ten produkt?
Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
K Tsutsui et al.
The Journal of biological chemistry, 268(25), 19076-19083 (1993-09-05)
cDNA segments for DNA topoisomerase II were amplified from rat brain RNA after reverse transcription by the polymerase chain reaction, using degenerate oligonucleotide primers deduced from the conserved regions of topoisomerase II of higher eukaryotes. The cDNA product from a
The effects of chemotherapy on morphology, cellular proliferation, apoptosis and oncoprotein expression in primary breast carcinoma.
Rasbridge, S A, et al.
British Journal of Cancer, 70, 335-341 (1994)
N I Valkov et al.
British journal of haematology, 108(2), 331-345 (2000-02-26)
The resistance of several leukaemic and myeloma cell lines (CCRF, L1210, HL-60, KG-1a and RPMI 8226) to VP-16 was found to increase with cell density and to be maximal (3.5- to 39-fold) in plateau phase cell cultures, as measured by
Ganglioglioma: a detailed histopathological and immunohistochemical analysis of 61 cases.
Wolf, H K, et al.
Acta neuropathologica, 88, 166-173 (1994)
KiS1--a novel monoclonal antibody which recognizes proliferating cells: evaluation of its relationship to prognosis in mammary carcinoma.
Sampson, S A, et al.
The Journal of Pathology, 168, 179-185 (1992)
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