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Key Documents

MAB3828

Sigma-Aldrich

Anti-Mucin 5B Antibody, clone 15.5B

clone 15.5B, Chemicon®, from mouse

Synonim(y):

MUC5B

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About This Item

Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.43

pochodzenie biologiczne

mouse

Poziom jakości

forma przeciwciała

purified immunoglobulin

rodzaj przeciwciała

primary antibodies

klon

15.5B, monoclonal

reaktywność gatunkowa

human, monkey

producent / nazwa handlowa

Chemicon®

metody

ELISA: suitable
immunohistochemistry: suitable
western blot: suitable

izotyp

IgG2b

numer dostępu NCBI

numer dostępu UniProt

Warunki transportu

wet ice

docelowa modyfikacja potranslacyjna

unmodified

informacje o genach

human ... MUC5B(727897)

Opis ogólny

Mucins are a family of highly glycosylated proteins that are present on the surface of, and are secreted by, many glandular epithelial cells. Mucins provide a barrier between the luminal membranes of cells in the epithelium and their environment. For instance, in the digestive tract, the mucin barrier protects the epithelial cells from the action of proteolytic enzymes. These high molecular weight glycoproteins consist of a nonglobular protein backbone (apomucin) and many O-linked glycans. Salivary Mucin 5B proteins are some of the largest polypeptides, with molecular weights ranging from 14 - 40 x 103 kDa.

Specyficzność

Reacts with Mucin 5B (MUC5B). Does not react with human Mucin 5A or 5C.

Immunogen

Un-glycosylated, serine- and threonine-free synthetic peptide from the central region of human MUC5B.

Zastosowanie

Research Category
Apoptosis & Cancer
Research Sub Category
Tumor Markers
This Anti-Mucin 5B Antibody, clone 15.5B is validated for use in ELISA, WB, IH for the detection of Mucin 5B.
Western blot: Bands in the high molecular weight range of 200kDa or above are expected for highly glycosylated proteins.

Immunohistchemistry: Paraffin sections. Deglycosylating the tissue is critical to expose epitope. There are a number of deglycosylation protocols available and a discussion of these related to Mucin can be found in Cao, Y. et al. (1997)(see references).

ELISA

Optimal working dilutions must be determined by end user.

Postać fizyczna

Format: Purified
Purified immunoglobulin. Liquid in PBS containing 0.1% sodium azide.

Przechowywanie i stabilność

Maintain at 2-8°C in undiluted aliquots for up to 6 months after date of receipt.

Inne uwagi

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informacje prawne

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania

10 - Combustible liquids

Klasa zagrożenia wodnego (WGK)

WGK 2

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Mucin5B expression by lung alveolar macrophages is increased in long-term smokers.
Sepper, R; Prikk, K; Metsis, M; Sergejeva, S; Pugatsjova, N; Bragina, O; Marran, S; Fehniger, TE
Journal of Leukocyte Biology null
Reduced thioredoxin increases proinflammatory cytokines and neutrophil influx in rat airways: modulation by airway mucus.
Raymond C Rancourt, Rees L Lee,
Free radical biology & medicine null
D J Thornton et al.
Glycobiology, 9(3), 293-302 (1999-02-20)
The MG1 population of mucins was isolated from human whole salivas by gel chromatography followed by isopycnic density gradient centrifugation. The reduced and alkylated MG1 mucins, separated by anion exchange chromatography, were of similar size (radius of gyration 55-64 nm)
Kristina A Thomsson et al.
Glycobiology, 12(1), 1-14 (2002-02-05)
The high-molecular-mass salivary mucin MG1, one of two major mucins produced by human salivary glands, plays an important role in oral health by coating the tooth surface and by acting as a bacterial receptor. Here this mucin was purified from
Mucins (MUC1 and MUC3) of gastrointestinal and breast epithelia reveal different and heterogeneous tumor-associated aberrations in glycosylation
Cao, Y, et al
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 45, 1547-1557 (1997)

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