MAB367
Anti-Muscarinic Acetylcholine Receptor m2 Antibody, clone M2-2-B3
clone M2-2-B3, Chemicon®, from rat
Synonim(y):
Przeciwciało przeciwko receptorowi muskarynowemu
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About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Polecane produkty
pochodzenie biologiczne
rat
Poziom jakości
forma przeciwciała
purified immunoglobulin
rodzaj przeciwciała
primary antibodies
klon
M2-2-B3, monoclonal
reaktywność gatunkowa
human, monkey, rat
reaktywność gatunkowa (przewidywana na podstawie homologii)
mammals
producent / nazwa handlowa
Chemicon®
metody
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
izotyp
IgG2a
przydatność
not suitable for immunohistochemistry (Paraffin)
numer dostępu NCBI
numer dostępu UniProt
Warunki transportu
wet ice
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
human ... CHRM2(1129)
Specyficzność
m2 muscarinic acetylcholine receptor. No reactivity with the other subtypes.
SPECIES REACTIVITIES:
Expected to react with most mammalian species.
SPECIES REACTIVITIES:
Expected to react with most mammalian species.
Immunogen
i3 loop of m2 receptor fusion protein (225-359), fused to Glutathione S-transferase.
Zastosowanie
Anti-Muscarinic Acetylcholine Receptor m2 Antibody, clone M2-2-B3 is an antibody against Muscarinic Acetylcholine Receptor m2 for use in IC, IH & IP.
Immunohistochemistry on 4% paraformaldehyde fixed tissue. Does not work on paraffin embedded tissues. Suggested starting concentration 1-5 μg/mL. It is suggested that you use the PAP system if using this antibody on rat. Immunocytochemistry on transfected cells
Immunoprecipitation Works poorly for immunoblotting Optimal working dilutions must be determined by end user.
IMMUNOHISTOCHEMISTRY PROTOCOL FOR MAB367
This antibody has been used successfully on 30 mm, free floating, 4% paraformaldehyde fixed rat brain tissue. All steps are performed under constant agitation. Suggested protocol follows.
1) 3 x 10 minute washes in TBS (with or without 0.25% Triton).
2) Incubate for 30 minutes in TBS with 3% serum (same as host from secondary antibody).
3) Incubate primary antibody diluted appropriately in TBS with 1% serum (same as host from secondary antibody) (with or without 0.25% Triton) for 2 hours at room temperature followed by 16 hours at 4°C.
4) 3 x 10 minute washes in TBS.
5) Incubate with secondary antibody diluted appropriately in TBS with 1% serum (same as host from secondary antibody).
6) 3 x 10 minute washes in TBS.
7) ABC Elite (1:200 Vector Labs) in TBS.
8) 2 x 10 minute washes in TBS.
9) 1 x 10 minute wash in phosphate buffer (no saline).
10) DAB reaction with 0.06% NiCl added for intensification.
11) 2 x 10 minute washes in PBS.
12) 1 x 10 minute wash in phosphate buffer (no saline).
Immunoprecipitation Works poorly for immunoblotting Optimal working dilutions must be determined by end user.
IMMUNOHISTOCHEMISTRY PROTOCOL FOR MAB367
This antibody has been used successfully on 30 mm, free floating, 4% paraformaldehyde fixed rat brain tissue. All steps are performed under constant agitation. Suggested protocol follows.
1) 3 x 10 minute washes in TBS (with or without 0.25% Triton).
2) Incubate for 30 minutes in TBS with 3% serum (same as host from secondary antibody).
3) Incubate primary antibody diluted appropriately in TBS with 1% serum (same as host from secondary antibody) (with or without 0.25% Triton) for 2 hours at room temperature followed by 16 hours at 4°C.
4) 3 x 10 minute washes in TBS.
5) Incubate with secondary antibody diluted appropriately in TBS with 1% serum (same as host from secondary antibody).
6) 3 x 10 minute washes in TBS.
7) ABC Elite (1:200 Vector Labs) in TBS.
8) 2 x 10 minute washes in TBS.
9) 1 x 10 minute wash in phosphate buffer (no saline).
10) DAB reaction with 0.06% NiCl added for intensification.
11) 2 x 10 minute washes in PBS.
12) 1 x 10 minute wash in phosphate buffer (no saline).
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
Neurotransmitters & Receptors
Postać fizyczna
Format: Purified
Purified immunoglobulin. Liquid in 0.02 M phosphate buffer, 0.25 M NaCl with 0.1% sodium azide, pH 7.6.
Przechowywanie i stabilność
Maintain at 2-8°C in undiluted aliquots for up to 6 months.
Inne uwagi
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informacje prawne
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania
10 - Combustible liquids
Klasa zagrożenia wodnego (WGK)
WGK 2
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
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