MAB1949
Anti-Laminin-5 Antibody, clone P3E4
clone P3E4, Chemicon®, from mouse
Synonim(y):
Epiligrin
Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych
About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Polecane produkty
pochodzenie biologiczne
mouse
Poziom jakości
forma przeciwciała
purified antibody
rodzaj przeciwciała
primary antibodies
klon
P3E4, monoclonal
reaktywność gatunkowa
human
producent / nazwa handlowa
Chemicon®
metody
ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
izotyp
IgG1
przydatność
not suitable for activity/function inhibition
numer dostępu NCBI
numer dostępu UniProt
Warunki transportu
wet ice
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
human ... LAMC2(3918)
Opis ogólny
Epiligrin, the major component of human keratinocyte extracellular matrix, serves as the preferred integrin ligand for alpha 3 beta 1 in plasma membranes and focal adhesions, and colocalizes with alpha 6 beta 4 in hemidesmosomes. {Domloge-Hultsch, et al (1992) J Clin Invest 90(4):1628-1633}. Major glycoprotein of epidermal basement membrane, consisting of three disulphide bonded subunits of 170, 145 and 135 kD. Epiligrin is the major ligand for alpha3/beta1 integrin, is particularly prominent in the lamina lucida of the skin and is absent in patients with lethal junctional epidermolysis bullosa. Today, epiligrin is identical to laminin 5, also called BM600, nicein, and kalinin. Laminin 5 (epiligrin; Carter et al, 1991 Cell 65(4):599-610) as the primary ligand in epithelial BMs that mediates both cell anchorage (adhesion without migration) via integrin a6b4 in homeostatic tissue and cell migration via integrin a3b1 in wound repair.
The laminin-5 isoform (nicein, epiligrin, and kalinin) is abundant in transitional epithelium, stratified squamous epithelia, lung mucosa, and other epithelial glands (Kallunki et al., 1992; Stahl et al., 1997). Laminin-5 is a heterotrimer consisting of alpha3, beta3, and gamma2 subunits that associate via large helical regions to produce a cruciform-shaped molecule (Rousselle et al., 1991 J. Cell Biol. 114: 567-576; Baker et al., 1996 J. Cell Sci. 109: 2509-2520). Laminin-5 is synthesized initially as a 460-kD molecule that undergoes specific processing to a smaller form after being secreted into the extracellular matrix (Marinkovich et al., 1992 J. Biol. Chem. 267: 17900-17906; Vailly et al., 1994 Eur. J. Biochem. 219: 209-218; Matsui et al., 1995 J. Biol. Chem. 270: 23496-23503). The size reduction is a result of processing the 3 and 2 subunits from 190-200 to 160 kD and from 155 to 105 kD, respectively (Marinkovich et al., 1992; Vailly et al., 1994; Matsui et al., 1995). {Goldfinger, LE (1998) J Cell Biol 141(1):255-265}.
The laminin-5 isoform (nicein, epiligrin, and kalinin) is abundant in transitional epithelium, stratified squamous epithelia, lung mucosa, and other epithelial glands (Kallunki et al., 1992; Stahl et al., 1997). Laminin-5 is a heterotrimer consisting of alpha3, beta3, and gamma2 subunits that associate via large helical regions to produce a cruciform-shaped molecule (Rousselle et al., 1991 J. Cell Biol. 114: 567-576; Baker et al., 1996 J. Cell Sci. 109: 2509-2520). Laminin-5 is synthesized initially as a 460-kD molecule that undergoes specific processing to a smaller form after being secreted into the extracellular matrix (Marinkovich et al., 1992 J. Biol. Chem. 267: 17900-17906; Vailly et al., 1994 Eur. J. Biochem. 219: 209-218; Matsui et al., 1995 J. Biol. Chem. 270: 23496-23503). The size reduction is a result of processing the 3 and 2 subunits from 190-200 to 160 kD and from 155 to 105 kD, respectively (Marinkovich et al., 1992; Vailly et al., 1994; Matsui et al., 1995). {Goldfinger, LE (1998) J Cell Biol 141(1):255-265}.
Specyficzność
Human epiligrin (laminin 5)
Immunogen
Human keratinocytes
Zastosowanie
Immunohistochemistry: for use on acetone fixed tissue
Immunocytochemistry
Immunoblotting: (non-reducing)
Immunoprecipitation
ELISA
Optimal working dilutions must be determined by end user.
Immunocytochemistry
Immunoblotting: (non-reducing)
Immunoprecipitation
ELISA
Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
ECM Proteins
ECM Proteins
This Anti-Laminin-5 Antibody, clone P3E4 is validated for use in ELISA, IP, WB, IC, IH for the detection of Laminin-5.
Postać fizyczna
Format: Purified
Please see datasheet for lot-specific information.
Przechowywanie i stabilność
Store at +2 to 8°C.
Inne uwagi
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informacje prawne
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania
12 - Non Combustible Liquids
Klasa zagrożenia wodnego (WGK)
WGK 1
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Normalized proliferation of normal and psoriatic keratinocytes by suppression of sAPPalpha-release.
Siemes, C; Quast, T; Klein, E; Bieber, T; Hooper, NM; Herzog, V
The Journal of Investigative Dermatology null
P Sriramarao et al.
The Journal of biological chemistry, 268(29), 22036-22041 (1993-10-15)
The purpose of this study was to determine if the alpha 3 beta 1 integrin could interact in a homophilic manner. Several earlier reports have shown that certain integrin adhesion receptors, namely alpha 2 beta 1, alpha 3 beta 1
M Barzegar et al.
The British journal of dermatology, 169(6), 1353-1356 (2013-07-23)
Laryngo-onycho-cutaneous (LOC) syndrome is a subtype of autosomal recessive junctional epidermolysis bullosa in which there is prominent skin and mucosal granulation tissue that can lead to delayed wound healing, laryngeal obstruction and blindness. Thus far, all cases are of Punjabi
Fibroblasts facilitate re-epithelialization in wounded human skin equivalents.
El Ghalbzouri, Abdoelwaheb, et al.
Laboratory Investigation; a Journal of Technical Methods and Pathology, 84, 102-112 (2004)
U M Wewer et al.
Laboratory investigation; a journal of technical methods and pathology, 71(5), 719-730 (1994-11-01)
The laminins are heterotrimeric basement membrane glycoproteins. Eight subunits that can be assembled into laminins have been characterized and are known as: A, B1, B2, S, M, K, B2t, B1k laminin chains. Although many neoplastic cells secrete laminins and some
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