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MAB16985

Sigma-Aldrich

Anti-MCAM Antibody, clone P1H12

clone P1H12, Chemicon®, from mouse

Synonim(y):

CD146 antigen, Cell surface glycoprotein P1H12, Melanoma-associated antigen A32, Melanoma-associated antigen MUC18, S-endo 1 endothelial-associated antigen, melanoma adhesion molecule, melanoma cell adhesion molecule

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About This Item

Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
klon:
P1H12, monoclonal
application:
ELISA
FACS
ICC
IHC
IP
WB
reaktywność gatunkowa:
mouse, canine, human
metody:
ELISA: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
citations:
20

pochodzenie biologiczne

mouse

Poziom jakości

100

forma przeciwciała

purified immunoglobulin

rodzaj przeciwciała

primary antibodies

klon

P1H12, monoclonal

reaktywność gatunkowa

mouse, canine, human

spodziewany brak reakcji z

rat

opakowanie

antibody small pack of 25 μg

producent / nazwa handlowa

Chemicon®

metody

ELISA: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

izotyp

IgG1

przydatność

not suitable for immunohistochemistry (Paraffin)

numer dostępu NCBI

NM_006500.2

numer dostępu UniProt

P43121

Warunki transportu

ambient

temp. przechowywania

2-8°C

docelowa modyfikacja potranslacyjna

unmodified

informacje o genach

human ... MCAM(4162)

Powiązane kategorie

Opis ogólny

MUC18 antigen (CD146), a member of the immuoglobulin superfamily, is an intrinsic membrane glycoprotein of 110-120 kDa found on the surface of endothelial cells, bone marrow fibroblasts and various melanomas. MUC18 has been used as a marker of tumor progression in human melanoma because expression in those tumors correlates strongly with poor prognosis and the development of metastic disease. In addition, a number of human T, B and myeloid leukemic cell lines seem to express MUC18. The close structural relationship with N-CAM and related molecules suggests that MUC18 may be also a developmentally regulated cell adhesion molecule (Melanoma adhesion molecule or MCAM).

Specyficzność

Detects circulating endothelial cells in human. Expected to cross-react with dog & mouse.

Immunogen

Immunization of mice with HUVEC.

Zastosowanie

Anti-MCAM Antibody, clone P1H12 detects level of MCAM & has been published & validated for use in ELISA, FC, IC, IH, IP & WB.
Immunocytochemistry:
1-10 μg/mL of a previous lot worked in immunocytochemistry.Works best on EDTA or Trypsin lifted endothelial cells.

Immunohistochemistry:
1-10 µg/mL. 4% PFA for 30min RT or <2hrs at 4°C. Block w/ 1%BSA/0.2% tween20/PBS for 30min. Works well in frozen tissue; fixed or unfixed.

Immunoprecipitation:
1-10 μg/mL of a previous lot worked in immunoprecipitation.

ELISA:
1-10 μg/mL of a previous lot worked in ELISA.

FACS Analysis:
1-10 μg/mL of a previous lot worked in FACS.

Optimal working dilutions must be determined by end user.

Jakość

Routinely evaluated by Western Blot on HUVEC lysates.

Western Blot Analysis:
1:500 dilution of this lot detected MCAM on 10μg of HUVEC lysates.

Opis wartości docelowych

110 kDa

Postać fizyczna

Format: Purified
Purified mouse monoclonal IgG1 in buffer containing 0.02 M PB with 0.25 M NaCl, pH=7.6, with 0.1% sodium azide.

Komentarz do analizy

Control
HUVEC cells.

Inne uwagi

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informacje prawne

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

WGK 2

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Keiich Hirono et al.
Journal of the American College of Cardiology, 48(6), 1257-1264 (2006-09-19)
This study investigated patients with acute Kawasaki disease (KD) to validate myeloid-related protein (MRP)-8/MRP-14 as a marker of disease activity and severity of coronary artery lesion development. Both MRP-8 and -14, which are S100-proteins secreted by activated neutrophils and monocytes
The assessment of CD146-based cell sorting and telomere length analysis for establishing the identity of mesenchymal stem cells in human umbilical cord.
Kouroupis, D; Churchman, SM; McGonagle, D; Jones, EA
F1000Research null
Differential distribution of stem cells in the auditory and vestibular organs of the inner ear.
Oshima, K; Grimm, CM; Corrales, CE; Senn, P; Martinez Monedero, R; Geleoc, GS; Edge et al.
Journal of the Association for Research in Otolaryngology : JARO null
D Baeten et al.
Annals of the rheumatic diseases, 63(5), 489-493 (2004-04-15)
It has been suggested that the immunopathology of rheumatoid nodules parallels that of inflamed synovium in rheumatoid arthritis (RA). To analyse the effect of infliximab on the immunopathology of rheumatoid nodules in order to provide new insights into the relationship
Ariya D Lapan et al.
Methods in molecular biology (Clifton, N.J.), 798, 3-19 (2011-12-02)
Dissociated human fetal skeletal muscle contains myogenic cells, as well as non-myogenic cells such as adipocytes, fibroblasts, and lymphocytes. It is therefore important to determine an efficient and reliable isolation method to obtain a purer population of myoblasts. Toward this
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