MAB1059
Anti-Pigment Epithelium Derived Factor Antibody, clone 10F12.2
clone 10F12.2, Chemicon®, from mouse
Synonim(y):
pigment epithelium-derived factor, proliferation-inducing protein 35, serine (or cysteine) proteinase inhibitor, clade F (alpha-2
antiplasmin, pigment epithelium derived factor), member 1, serpin peptidase inhibitor, clade F (alpha-2 antiplasmin
Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych
About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Polecane produkty
pochodzenie biologiczne
mouse
Poziom jakości
forma przeciwciała
purified immunoglobulin
rodzaj przeciwciała
primary antibodies
klon
10F12.2, monoclonal
reaktywność gatunkowa
human
reaktywność gatunkowa (przewidywana na podstawie homologii)
rat
producent / nazwa handlowa
Chemicon®
metody
ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
izotyp
IgG2a
numer dostępu NCBI
numer dostępu UniProt
Warunki transportu
wet ice
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
human ... SERPINF1(5176)
Opis ogólny
Pigment epithelium-derived factor (PEDF) was first identified as a 50 kDa secreted protein in conditioned medium from cultured fetal human retinal pigment epithelium (RPE) cells (Tombran-Tink, 1991). More recently, PEDF was shown to have neurotrophic activity in the retina in vivo (Cayouette, 1999). PEDF is also a neurotrophic and neuroprotective factor in other systems, including cultured cerebellar granule cells, primary hippocampal neurons, spinal cord motor neurons and amphibian photoreceptors cultured in the absence of RPE cells (Karakousis, 2001). The binding of PEDF to retinoblastoma and cerebellar granule cells may be receptor-mediated. Recently, PEDF was shown to be a potent inhibitor of angiogenesis in endothelial cell cultures and in rat cornea and retina in vivo (Dawson, 1999; Stellmach, 2001).
Specyficzność
Recognizes Pigment-Epithelium Derived Factor (PEDF).
Immunogen
Recombinant human GST-PEDF fusion protein. The sequence corresponding to amino
acids 196-347 of human PEDF tagged with GST on the N-terminal was expressed in E. Coli. GST-PEDF is about 46 kDa on SDS-PAGE.
acids 196-347 of human PEDF tagged with GST on the N-terminal was expressed in E. Coli. GST-PEDF is about 46 kDa on SDS-PAGE.
Zastosowanie
Anti-Pigment Epithelium Derived Factor Antibody, clone 10F12.2 is an antibody against Pigment Epithelium Derived Factor for use in ELISA, IC, IH & WB.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurochemistry & Neurotrophins
Neurochemistry & Neurotrophins
Western Blot:
Recognizes a protein with a molecular weight of ~46 kDa when tested against immunogen. Western blot positive control is available (Catalog number AG672)
ELISA:
A previous lot of this antibody was used on ELISA.
Immunocytochemistry:
A previous lot of this antibody was used on Immunocytochemistry.
Immunohistochemistry:
A previous lot of this antibody was used on frozen sections.
Optimal working dilutions must be determined by end user.
Recognizes a protein with a molecular weight of ~46 kDa when tested against immunogen. Western blot positive control is available (Catalog number AG672)
ELISA:
A previous lot of this antibody was used on ELISA.
Immunocytochemistry:
A previous lot of this antibody was used on Immunocytochemistry.
Immunohistochemistry:
A previous lot of this antibody was used on frozen sections.
Optimal working dilutions must be determined by end user.
Jakość
Routinely evaluated by Western Blot on human placenta lysate.
Western Blot Analysis:
1:1000 dilution of this lot detected pigment epithelium on 10 μg of human placenta lysate.
Western Blot Analysis:
1:1000 dilution of this lot detected pigment epithelium on 10 μg of human placenta lysate.
Opis wartości docelowych
~46 kDa
Postać fizyczna
Format: Purified
Protein A purified
Purified mouse monoclonal IgG2a in buffer containing 0.02 M PBS, pH 7.6, 0.25 M NaCl with 0.1% sodium azide.
Przechowywanie i stabilność
Stable for 6 months at 2-8°C in undiluted aliquots from date of receipt.
Komentarz do analizy
Control
Eye tissue.
Eye tissue.
Inne uwagi
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informacje prawne
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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polecane
Kod klasy składowania
10 - Combustible liquids
Klasa zagrożenia wodnego (WGK)
WGK 2
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
Masz już ten produkt?
Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Identification of pigment epithelium-derived factor as a direct target of the p53 family member genes.
Sasaki, Y; Naishiro, Y; Oshima, Y; Imai, K; Nakamura, Y; Tokino, T
Oncogene null
V Stellmach et al.
Proceedings of the National Academy of Sciences of the United States of America, 98(5), 2593-2597 (2001-02-28)
Aberrant blood vessel growth in the retina that underlies the pathology of proliferative diabetic retinopathy and retinopathy of prematurity is the result of the ischemia-driven disruption of the normally antiangiogenic environment of the retina. In this study, we show that
Shou-Chuan Shih et al.
PloS one, 11(7), e0157647-e0157647 (2016-07-08)
Fibrogenesis is induced by repeated injury to the liver and reactive regeneration and leads eventually to liver cirrhosis. Pigment epithelium derived factor (PEDF) has been shown to prevent liver fibrosis induced by carbon tetrachloride (CCl4). A 44 amino acid domain
Pigment epithelium-derived factor stimulates tumor macrophage recruitment and is downregulated by the prostate tumor microenvironment.
Halin, S; Rudolfsson, SH; Doll, JA; Crawford, SE; Wikstrom, P; Bergh, A
Neoplasia null
Pigment epithelium derived factor inhibits the growth of human endometrial implants in nude mice and of ovarian endometriotic stromal cells in vitro.
Sun, Y; Che, X; Zhu, L; Zhao, M; Fu, G; Huang, X; Xu, H; Hu, F; Zhang, X
Testing null
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