GR13L

Anti-EGFR (Ab-3) Mouse mAb (225)

lyophilized, clone 225, Calbiochem^®

• Synonim(y): Anti-EGF-R
• Kod UNSPSC: 12352203
• NACRES: NA.43

Właściwości

• pochodzenie biologiczne: mouse
• Poziom jakości: 100
• forma przeciwciała: purified antibody
• rodzaj przeciwciała: primary antibodies
• klon: 225, monoclonal
• Formularz: lyophilized
• nie zawiera: preservative
• reaktywność gatunkowa: human
• spodziewany brak reakcji z: rat, mouse
• producent / nazwa handlowa: Calbiochem^®
• warunki przechowywania: OK to freeze
• izotyp: IgG1
• Warunki transportu: ambient
• temp. przechowywania: 2-8°C
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: human ... EGFR(1956) ; mouse ... Egfr(13649) ; rat ... Egf(25313)

Opis

Opis ogólny

Anti-EGFR (Ab-3), mouse monoclonal, clone 225 recognizes the ~170 kDa epidermal growth factor receptor protein. It is validated for use in immunoprecipitation and neutralization.

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells (see application references). Recognizes the ~170 kDa epidermal growth factor receptor protein.

Recognizes the ~170 kDa epidermal growth factor receptor protein in A431 cells.

Immunogen

Human

partially purified, human EGFR from A431 cells

Zastosowanie

Immunoprecipitation (1-2 µg per sample)

Neutralization Studies (2 µg/ml, see comments and application references)

Ostrzeżenie

Toxicity: Standard Handling (A)

Postać fizyczna

Lyophilized from 20 mM ammonium bicarbonate solution, 100 µg BSA.

Rekonstytucja

Reconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml. Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate at 4°C with 0.1% azide for short-term storage. Avoid freeze/thaw cycles of solutions.

Komentarz do analizy

Negative Control
HL-60 cells

Positive Control
A431 cells

Inne uwagi

Hunter T. 1984. Nature311, 414.
Reynolds, F.H., et. al 1981. Nature292, 259.
Ushiro, H. and Cohen, S. 1980. J. Biol. Chem.255, 8363.

This antibody has been shown to inhibit human tumor xenographs in nude mice. It inhibits EGF binding to its receptor and is an antagonist of in vivo EGF-stimulated tyrosine kinase activity. It also inhibits A431 tumor formation in nude mice. Antibody should be titrated for optimal results in individual systems.

Informacje prawne

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 10 - Combustible liquids
• Klasa zagrożenia wodnego (WGK): nwg
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable