FCMAB115F
Anti-TRA-1-60 Antibody, clone TRA-1-60, FITC conjugate
clone TRA-1-60, from mouse, FITC conjugate
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About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
pochodzenie biologiczne
mouse
Poziom jakości
białko sprzężone
FITC conjugate
forma przeciwciała
purified antibody
rodzaj przeciwciała
primary antibodies
klon
TRA-1-60, monoclonal
reaktywność gatunkowa (przewidywana na podstawie homologii)
human (based on 100% sequence homology)
metody
flow cytometry: suitable
immunocytochemistry: suitable
izotyp
IgM
Warunki transportu
wet ice
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
human ... PODXL(5420)
Opis ogólny
Human embryonal carcinoma (EC) cells are the stem cells of teratocarcinomas, and they are key components of germ cell tumors (GCTs). They express several high molecular weight glycoprotein antigens that are down-regulated upon differentiation. One of these antigens, defined by monoclonal antibody TRA-1-60, can be detected in the serum of GCT patients and provides a useful complement to the established serum markers human chorionic gonadotropin and α-fetoprotein, especially in those patients without elevated serum human chorionic gonadotropin or α-fetoprotein.
Specyficzność
This antibody reacts with TRA-1-60 antigen that is expressed upon the surface of human tetracarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES).
No immunoreactivity is seen with murine EC, EG, or ES cells. Both the TRA-1-60 and TRA-1-81 monoclonal antibodies (MAB4381) recognize antigens that are associated with a pericellular matrix proteoglycan. TRA-1-60 reacts with a sialidase-sensitive epitope whilst TRA-1-81 reacts with an unknown epitope of the same molecule
No immunoreactivity is seen with murine EC, EG, or ES cells. Both the TRA-1-60 and TRA-1-81 monoclonal antibodies (MAB4381) recognize antigens that are associated with a pericellular matrix proteoglycan. TRA-1-60 reacts with a sialidase-sensitive epitope whilst TRA-1-81 reacts with an unknown epitope of the same molecule
Immunogen
- Whole Cells corresponding to the of TRA-1-60.
Zastosowanie
Detect TRA-1-60 using this Anti-TRA-1-60 Antibody, clone TRA-1-60, FITC conjugate validated for use in FC & IC.
Immunocytochemical staining of fixed H9 human ES cells incubated for two hours at 2-8C with 1:100 dilution of anti-TRA-1-60 FITC conjugated (Cat. No. FCMAB115F) monoclonal antibody. Pluripotent human ES cells exhibit strong immunoreactivity to this antibody.
Immunocytochemical staining of live H9 human ES cells incubated for 30 minutes at 37C with 1:100 dilution of anti-TRA-1-60 FITC conjugated (Cat. No. FCMAB115F) monoclonal antibody. Pluripotent human ES cells exhibit strong immunoreactivity to this antibody.
Flow Cytometry:
Antibody dilution for cellular staining:
5 μl Working Solution: 5 x 105 Cells / 95 μl PBS. 100 μl Total Reaction Volume
10 μl Working Solution: 1 x 106 Cells / 90 μl PBS. 100 μl Total Reaction Volume
Immunocytochemical staining of live H9 human ES cells incubated for 30 minutes at 37C with 1:100 dilution of anti-TRA-1-60 FITC conjugated (Cat. No. FCMAB115F) monoclonal antibody. Pluripotent human ES cells exhibit strong immunoreactivity to this antibody.
Flow Cytometry:
Antibody dilution for cellular staining:
- Prepare an antibody working solution by diluting 1:5 the primary antibody with PBS.
- Dispense the volume per test of working of solution according to the number of cells indicated in the table below.
- 5 L for Guava Flow Cytometer
- 10 L for other Flow Cytometry instruments
5 μl Working Solution: 5 x 105 Cells / 95 μl PBS. 100 μl Total Reaction Volume
10 μl Working Solution: 1 x 106 Cells / 90 μl PBS. 100 μl Total Reaction Volume
Research Category
Stem Cell Research
Stem Cell Research
Research Sub Category
Pluripotent & Early Differentiation
Pluripotent & Early Differentiation
Opis wartości docelowych
~235/410 kDa
Postać fizyczna
Purified mouse monoclonal IgM conjugated to FITC in PBS with less than 0.09% sodium azide and 15 mg/mL BSA.
Przechowywanie i stabilność
Maintain refrigerated at 2-8ºC protected from light in undiluted aliquots for up to 6 months from date of receipt.
Komentarz do analizy
Control
Pluripotent human embryonic stem (ES) cells
Pluripotent human embryonic stem (ES) cells
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania
10 - Combustible liquids
Klasa zagrożenia wodnego (WGK)
WGK 2
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Cathelijn E M Aarts et al.
Stem cell research, 54, 102444-102444 (2021-06-29)
Induced pluripotent stem cells (iPSCs) were generated from blood outgrowth endothelial cells (BOECs) obtained from a healthy donor and from a patient diagnosed with Hermansky Pudlak Syndrome type 2 (HPS2), caused by compound heterozygous AP3B1 mutations (c.177delA and c.1839-1842delTAGA). BOECs
Toshio Miki et al.
Stem cell research & therapy, 7, 8-8 (2016-01-14)
Amnion-derived stem cells have been proposed for cell replacement therapy and tissue regeneration. An easily accessible cell source, the placenta, allows us to potentially establish a bio-bank of cells for immunotype matched clinical applications. Several xeno-free (XF) cryopreservation media are
Passaging and colony expansion of human pluripotent stem cells by enzyme-free dissociation in chemically defined culture conditions.
Beers, J; Gulbranson, DR; George, N; Siniscalchi, LI; Jones, J; Thomson, JA; Chen, G
Nature Protocols null
Robert J Ihry et al.
Nature medicine, 24(7), 939-946 (2018-06-13)
CRISPR/Cas9 has revolutionized our ability to engineer genomes and conduct genome-wide screens in human cells1-3. Whereas some cell types are amenable to genome engineering, genomes of human pluripotent stem cells (hPSCs) have been difficult to engineer, with reduced efficiencies relative
Variability in the generation of induced pluripotent stem cells: importance for disease modeling.
Vitale, AM; Matigian, NA; Ravishankar, S; Bellette, B; Wood, SA; Wolvetang, EJ; Mackay-Sim, A
Stem Cells Translational Medicine null
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