ABC112

Anti-phospho-ULK1/ATG1 (Ser758) Antibody

from rabbit, purified by affinity chromatography

• Synonim(y): Serine/threonine-protein kinase ULK1, Autophagy-related protein 1 homolog, ATG1, hATG1, Unc-51-like kinase 1
• Kod UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: rabbit
• Poziom jakości: 100
• forma przeciwciała: affinity isolated antibody
• rodzaj przeciwciała: primary antibodies
• klon: polyclonal
• oczyszczone przez: affinity chromatography
• reaktywność gatunkowa: human
• reaktywność gatunkowa (przewidywana na podstawie homologii): bovine (based on 100% sequence homology), rat (based on 100% sequence homology), mouse (based on 100% sequence homology)
• metody: inhibition assay: suitable (peptide); western blot: suitable
• numer dostępu NCBI: NP_003556
• numer dostępu UniProt: O75385
• Warunki transportu: wet ice
• docelowa modyfikacja potranslacyjna: phosphorylation (pSer758)
• informacje o genach: human ... ULK1(8408)

Opis

Opis ogólny

ULK1 or autophagy-related protein 1 homolog (ATG1) is a serine/threonine protein kinase. It is a ubiquitously expressed protein that is highly abundant in the brain, skeletal muscle, and heart. It is involved in the signaling pathway of mTOR receptors leading to the regulation of autophagy in nutrient-deprived cells. mTOR may interact with ULK1 as a complex of ULK1/FIP200/Atg13. ULK1 may play an important role in neuronal development: Elimination of ULK1′s enzymatic activity by targeted disruption of its kinase domain has blocked the outgrowth of neurons in the developing mouse brain, possibly by blocking the ULK1-mediated trafficking of neurotrophins.

Specyficzność

This antibody recognizes the ULK1/ATG1 phosphorylated at Ser758.

Immunogen

Epitope: Phosphorylated Ser758

KLH-conjugated linear peptide corresponding to human ULK1/ATG1 phosphorylated at Ser758.

Zastosowanie

Detect phospho-ULK1/ATG1 (Ser758) using this Anti-phospho-ULK1/ATG1 (Ser758) Antibody validated for use in Western Blotting, Peptide Inhibition Assay.

Peptide Inhibition Analysis: A 1:1,000 dilution from a representative lot blocked phospho-ULK1/ATG1 (Ser758) in serum-starved A431 cell lysates.

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

Jakość

Evaluated by Western Blot in A431 and serum starved A431 cell lysates.

Western Blot Analysis: A 1:1,000 dilution of this antibody detected ULK1/ATG1 on 10 µg of A431 and serum starved A431 cell lysates..

Opis wartości docelowych

~120 kDa observed

Postać fizyczna

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Przechowywanie i stabilność

Stable for 1 year at 2-8°C from date of receipt.

Komentarz do analizy

Control
A431 and serum starved A431 cell lysates.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable