AB5062P
Anti-Vesicular GABA Transporter (VGAT) Antibody
Chemicon®, from rabbit
Synonim(y):
Vesicular Inhibitory Amino Acid Transporter
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About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Polecane produkty
pochodzenie biologiczne
rabbit
Poziom jakości
forma przeciwciała
affinity isolated antibody
rodzaj przeciwciała
primary antibodies
klon
polyclonal
oczyszczone przez
affinity chromatography
reaktywność gatunkowa
mouse, monkey, human, rat
producent / nazwa handlowa
Chemicon®
metody
ELISA: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable
numer dostępu NCBI
numer dostępu UniProt
Warunki transportu
dry ice
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
human ... SLC32A1(140679)
Opis ogólny
Vesicular GABA Transporter (unc-47) homologue is a 525 amino acid long protein belonging to the family of neurotransmitter transporter (1). VGAT functions in the uptake of GABA and glycine into synaptic vesicles. Since the protein is bound to synaptic vesicles, VGAT antibodies are excellent markers of presynaptic GABAergic and glycinergic neurons.
Specyficzność
Antibody cross-reactivity with various species is not known. The immunogen sequence is 100% conserved in mouse, human and monkey.
Specific for Vesicular GABA Transporter (VGAT). Vesicular GABA Transporter (unc-47) homologue is a 525 amino acid long protein belonging to the family of neurotransmitter transporter. The immunogen peptide shows no significant homology with other known proteins.
Immunogen
A 17 amino acid peptide sequence near the carboxy terminal region of rat VGAT (McIntire, S., 1997).
Zastosowanie
Immunohistochemistry/Immunofluorescence:
2-20 µg/mL of a previous lot was used on paraformaldehyde fixed sections.
ELISA:
1:10,000 – 1:100,000 dilution of a previous lot was used on ELISA, using 50-100 ng of control peptide (Cat. No. AG379) per well.
Western blot:
1-10 µg/mL (Chemiluminescence technique) The expected molecular weight is approximately 55-60 kDa.
Optimal working dilutions must be determined by the end user.
2-20 µg/mL of a previous lot was used on paraformaldehyde fixed sections.
ELISA:
1:10,000 – 1:100,000 dilution of a previous lot was used on ELISA, using 50-100 ng of control peptide (Cat. No. AG379) per well.
Western blot:
1-10 µg/mL (Chemiluminescence technique) The expected molecular weight is approximately 55-60 kDa.
Optimal working dilutions must be determined by the end user.
This Anti-Vesicular GABA Transporter (VGAT) Antibody is validated for use in ELISA, IF, IH, WB for the detection of Vesicular GABA Transporter (VGAT).
Jakość
Evaluated by Western Blot on Rat retina lysates.
Western Blot Analysis:
1:500 dilution of this antibody detected Vesicular GABA Transporter on 10μg of rat retina lysates.
Western Blot Analysis:
1:500 dilution of this antibody detected Vesicular GABA Transporter on 10μg of rat retina lysates.
Opis wartości docelowych
57 kDa
Postać fizyczna
Purified rabbit polyclonal in buffer containing PBS with 0.1% BSA.
Komentarz do analizy
Control
Brain tissue, rat retina lysate
Brain tissue, rat retina lysate
Inne uwagi
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informacje prawne
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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polecane
Kod klasy składowania
12 - Non Combustible Liquids
Klasa zagrożenia wodnego (WGK)
WGK 2
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
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Oum Kaltoum Hassani,Pablo Henny,Maan Gee Lee,Barbara E Jones
The European Journal of Neuroscience null
Characterization of axo-axonic synapses in the piriform cortex of Mus musculus.
Xinjun Wang,Qian-Quan Sun
The Journal of Comparative Neurology null
Cleavage of the vesicular GABA transporter under excitotoxic conditions is followed by accumulation of the truncated transporter in nonsynaptic sites.
Gomes, Jo?o R, et al.
The Journal of Neuroscience, 31, 4622-4635 (2011)
Phillip R Kramer et al.
Neuroscience, 356, 207-216 (2017-05-28)
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