AB3786

Anti-Prosurfactant Protein C (proSP-C) Antibody

serum, Chemicon^®

• Synonim(y): proSP-C
• Kod UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: rabbit
• Poziom jakości: 100
• forma przeciwciała: serum
• rodzaj przeciwciała: primary antibodies
• klon: polyclonal
• reaktywność gatunkowa: mouse, rat
• producent / nazwa handlowa: Chemicon^®
• metody: immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• numer dostępu NCBI: NM_003018.3
• numer dostępu UniProt: P11686
• Warunki transportu: dry ice
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: human ... SFTPC(6440) , SP5(389058)

Opis

Opis ogólny

Pulmonary surfactant is a complex mixture of phospholipids and proteins secreted from airway epithelial cells (type II cells) in alveoli which reduces the surface tension at the alveolar air liquid interface, providing alveolar stability necessary for normal ventilation. Four distinct proteins isolated from pulmonary surfactant are termed surfactant proteins A, B, C and D. SP A (28 - 36 kDa) and SP D (43 kDa) are collagenous carbohydrate binding proteins, whereas SP B (8 - 9 kDa) and SP C (4 kDa) are non collagenous hydrophobic proteins. SP C is a fragment of a much larger precursor protein of 21 kDa. The precursor contains an exceedingly hydrophobic region of 34 amino acids that comprises most of the mature SP C, and a unique poly-valine domain.

Specyficzność

Expected to cross-react with human.

Reacts strongly with proSP-C from human and mouse. Immunoprecipitates the 21 kDa proprotein as well as processing intermediates of M.W. 26 kDa, 21 kDa, 16 kDa and 14 kDa. Preincubation of the antiserum with the fusion protein results in ablation of immunostaining. On human tissue immunostains alveolar Type II cells in normal lung. The antibody also recognizes the aberrant form of proSP-C (6-12 kDa peptide) found in BAL and in alveolar proteinosis material on sections of lung samples from human patients with hereditary SP-B deficiency. On mouse tissue immunostains alveolar Type II cells in normal lung.

Immunogen

GST-tagged recombinant protein corresponding to the N-terminal region of human Prosurfactant Protein C (proSP-C).

Zastosowanie

• Western Blotting Analysis: A 1:500 dilution from a representative lot detected Prosurfactant Protein C (proSP-C) in mouse lung tissue lysate and GST-fusion Prosurfactant Protein C fragment.
• ELISA Analysis: A 1:50,000 dilution from a representative lot detected GST-fusion Prosurfactant Protein C fragment.
• Immunohistochemistry (Paraffin) Analysis: A 1:2,000 dilution from a representative lot detected Prosurfactant Protein C (proSP-C) in mouse lung tissue sections.
• Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Research Category
Metabolism

Research Sub Category
Ion & Transport Channels

This rabbit polyclonal Anti-Prosurfactant Protein C (proSP-C), Cat. No. AB3786 is tested for use in Western Blotting, Immunohistochemistry (Paraffin) and ELISA, for the detection of Prosurfactant Protein C (proSP-C).

Jakość

Evaluated by Western Blotting in rat lung tissue lysate. Western Blotting Analysis: A 1:500 dilution of this antibody detected Prosurfactant Protein C (proSP-C) in rat lung tissue lysate.

Opis wartości docelowych

20 kDa observed. 21 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Powiązanie

Replaces: 07-647

Postać fizyczna

Rabbit polyclonal antibody in serum with 0.05% sodium azide.

Unpurified

Przechowywanie i stabilność

Stable at -20ºC in undiluted aliquots for up to twelve months after date of receipt. Avoid repeated freeze/thaw cycles.

Komentarz do analizy

Control
Mouse lung epithelial cells.

Inne uwagi

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informacje prawne

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable