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AB2237

Sigma-Aldrich

Anti-PAX6 Antibody

from rabbit, purified by affinity chromatography

Synonim(y):

Aniridia type II protein, paired box 6, paired box gene 6, paired box gene 6 (aniridia, keratitis), paired box homeotic gene-6

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About This Item

Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

pochodzenie biologiczne

rabbit

Poziom jakości

100

forma przeciwciała

affinity isolated antibody

rodzaj przeciwciała

primary antibodies

klon

polyclonal

oczyszczone przez

affinity chromatography

reaktywność gatunkowa

mouse, rat, chicken

spodziewany brak reakcji z

human

metody

immunocytochemistry: suitable
western blot: suitable

numer dostępu NCBI

NM_013627

numer dostępu UniProt

P26367

Warunki transportu

wet ice

docelowa modyfikacja potranslacyjna

unmodified

informacje o genach

human ... PAX6(5080)

Opis ogólny

PAX6 is the most researched of the PAX genes and appears throughout the literature as a "master control" gene for the development of eyes and other sensory organs, certain neural and epidermal tissues as well as other homologous structures, usually derived from ectodermal tissues. This transcription factor is most famous for its use in the interspecifically induced expression of ectopic eyes and is of medical importance because heterozygous mutants produce a wide spectrum of ocular defects such as Aniridia in humans. This gene encodes paired box gene 6, one of many human homologues of the Drosophila melanogaster gene prd. In addition to the hallmark feature of this gene family, a conserved paired box domain, the encoded protein also contains a homeo box domain. Both domains are known to bind DNA, and function as regulators of gene transcription. This gene is expressed in the developing nervous system, and in developing eyes. Mutations in this gene are known to cause aniridia as well as Peter′s anomaly, both ocular diseases.

Specyficzność

This antibody recognizes the C-Terminus of PAX6.

Immunogen

KLH conjugated followed by PAX6 corresponding to the C-Terminus

Zastosowanie

Anti-PAX6 Antibody detects level of PAX6 & has been published & validated for use in WB & IC.
Immunocytochemistry:
Working dilutions of 1:50 - 1:1,000 of Anti-Pax6 was used to resolve the protein in chicken spinal cord.

Jakość

Routinely evaluated by Western Blot on rat and mouse brain lysates.

Western Blot Analysis:
0.5 µg/mL of this lot detected PAX6 on 10 µg of mouse and rat brain lysates.

Opis wartości docelowych

~48 kDa

Powiązanie

Replaces: AB5409

Postać fizyczna

Immunoaffinity purified rabbit polyclonal antibody in buffer containing 0.1M Tris-Glycine (pH7.4) 150mM NaCl with 0.05% NaN3.

Komentarz do analizy

Control
Mouse and rat brain lysates

Inne uwagi

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
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Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Certyfikaty analizy (CoA)

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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Jovica Ninkovic et al.
Neuron, 68(4), 682-694 (2010-11-26)
Most neurons in the adult mammalian brain survive for the entire life of an individual. However, it is not known which transcriptional pathways regulate this survival in a healthy brain. Here, we identify a pathway regulating neuronal survival in a
Keith W Vance et al.
The EMBO journal, 33(4), 296-311 (2014-02-04)
Although some long noncoding RNAs (lncRNAs) have been shown to regulate gene expression in cis, it remains unclear whether lncRNAs can directly regulate transcription in trans by interacting with chromatin genome-wide independently of their sites of synthesis. Here, we describe
Notch signaling maintains neural rosette polarity.
Main, H; Radenkovic, J; Jin, SB; Lendahl, U; Andersson, ER
Testing null
Erin Knock et al.
Neural development, 10, 13-13 (2015-05-03)
Chromatin-modifying complexes have key roles in regulating various aspects of neural stem cell biology, including self-renewal and neurogenesis. The methyl binding domain 3/nucleosome remodelling and deacetylation (MBD3/NuRD) co-repressor complex facilitates lineage commitment of pluripotent cells in early mouse embryos and
Yury Kiselev et al.
Scientific reports, 8(1), 5059-5059 (2018-03-24)
Lung cancer is the leading cause of cancer deaths. Novel predictive biomarkers are needed to improve treatment selection and more accurate prognostication. PAX6 is a transcription factor with a proposed tumour suppressor function. Immunohistochemical staining was performed on tissue microarrays
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