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Key Documents

386032

Sigma-Aldrich

Anti-Hsp70 Mouse mAb (C92F3A-5)

liquid, clone C92F3A-5, Calbiochem®

Synonim(y):

Anti-Heat Shock Protein 70

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About This Item

Kod UNSPSC:
12352203
NACRES:
NA.41

pochodzenie biologiczne

mouse

Poziom jakości

forma przeciwciała

purified antibody

rodzaj przeciwciała

primary antibodies

klon

C92F3A-5, monoclonal

Postać

liquid

zawiera

≤0.1% sodium azide as preservative

reaktywność gatunkowa (przewidywana na podstawie homologii)

all

producent / nazwa handlowa

Calbiochem®

warunki przechowywania

OK to freeze
avoid repeated freeze/thaw cycles

izotyp

IgG1

Warunki transportu

wet ice

temp. przechowywania

−20°C

docelowa modyfikacja potranslacyjna

unmodified

informacje o genach

human ... HSPA1A(3303)

Opis ogólny

Anti-Hsp70, mouse monoclonal, clone C92F3A-5, recognizes the ~70 kDa Hsp70. Does not cross-react with Hsc70. It is validated for use in ELISA, FC, WB, ICC, IP & IHC (frozen and paraffin sections).
Mouse monoclonal antibody purified by ion-exchange chromatography. Recognizes the ~70 kDa Hsp70 protein.
Recognizes the ~70 kDa Hsp70 protein. Does not cross-react with Hsc70.

Immunogen

Hsp70 from HeLa cells
Human

Zastosowanie

ELISA (see comments)

Flow Cytometry (see comments)

Frozen Sections (5 µg/ml)

Immunoblotting (1 µg/ml)

Immunocytochemistry (5 µg/ml)

Immunoprecipitation (see comments)

Paraffin Sections (5 µg/ml)

Opakowanie

Please refer to vial label for lot-specific concentration.

Ostrzeżenie

Toxicity: Standard Handling (A)

Postać fizyczna

In PBS, 50% glycerol, pH 7.2.

Rekonstytucja

Following iniital thaw, aliquot and freeze (-20°C).

Komentarz do analizy

Positive Control
L929 cells, Human colon cancer tissue

Inne uwagi

Does not cross-react with Hsc70. Because Hsp70 is not expressed constitutively in most cells, this antibody is suitable for ascertaining whether a stress-response has occurred in the cell. Increased levels of Hsp70 expression occurs following stress even in cells that constitutively express Hsp70. This antibody has also been reported to work for ELISA, flow cytometry, and immunoprecipitation. Variables associated with assay conditions will dictate the optimal working dilution.
Hang, H., and Fox, M.H. 1995. Cytometry19, 119.
Kilgore, J.L., et al. 1994. J. Appl. Physiol.76, 589.
Heufelder, A.E., et al. 1992. J. Clin. Endocrinol. Metab.74, 724.
Gower, D.J., et al. 1989. J. Neurosurg.70, 605.
Milarski, K., et al. 1989.J. Cell Biol.108, 413.
Vass, K., et al. 1988. Acta Neuropathologica77, 128.
Welch, W.J., and Suhan, J.P. 1986. J. Cell Biol.103, 2035.

Informacje prawne

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Kod klasy składowania

10 - Combustible liquids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Aigang Lu et al.
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism, 22(2), 183-195 (2002-02-02)
Estradiol reduces brain injury from many diseases, including stroke and trauma. To investigate the molecular mechanisms of this protection, the effects of 17-beta-estradiol on heat shock protein (HSP) expression were studied in normal male and female rats and in male
Anne K Voss et al.
The EMBO journal, 25(15), 3652-3663 (2006-07-22)
The mechanisms regulating the size of the cerebral cortex are poorly understood. Here, we demonstrate that the Rap1 guanine nucleotide exchange factor, C3G (Grf2, Rapgef1), controls the size of the cerebral precursor population. Mice lacking C3G show overproliferation of the
Kamlesh K Bhopale et al.
Alcoholism, clinical and experimental research, 41(10), 1675-1685 (2017-08-10)
Chronic alcohol abuse, a major risk factor for such diseases as hepatitis and cirrhosis, impairs hepatic alcohol dehydrogenase (ADH; key ethanol [EtOH]-metabolizing enzyme). Therefore, differentially altered hepatic and plasma proteomes were identified in chronic EtOH feeding model of hepatic ADH-deficient
Sara Iachettini et al.
Cell death & disease, 9(10), 996-996 (2018-09-27)
Sirtuin 6 (SIRT6) is a member of the NAD+-dependent class III deacetylase sirtuin family, which plays a key role in cancer by controlling transcription, genome stability, telomere integrity, DNA repair, and autophagy. Here we analyzed the molecular and biological effects
Amanda M Gleixner et al.
Molecular pharmacology, 92(5), 564-575 (2017-08-24)
N-acetyl-l-cysteine (NAC) exhibits protective properties in brain injury models and has undergone a number of clinical trials. Most studies of NAC have focused on neurons. However, neuroprotection may be complemented by the protection of astrocytes because healthier astrocytes can better

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