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Merck

23479

Sigma-Aldrich

Nitrite/Nitrate Assay Kit, colorimetric

used for detection of nitric oxide metabolites

Synonim(y):

Nitrate/Nitrite Assay Kit, colorimetric

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About This Item

Kod UNSPSC:
12161503
NACRES:
NA.84

Poziom jakości

zastosowanie

sufficient for 100 assays

przydatność

passes test for application

temp. przechowywania

2-8°C

Zastosowanie

Nitric oxide (NO), a gaseous paramagnetic radical, is a very important and versatile messenger in biological systems. NO is synthesized from L-arginine by NO synthase (NOS). It has been identified as an endothelial derived relaxation factor (EDRF) and antiplatelet substance. It serves as a neurotransmitter derived from a neutrophile and a cytotoxic substance from an activated macrophage. Although NO′s molecular action in the biological system is very versatile, the most important role of NO is the activation of guanylate cyclase.

The Griess assay is one of the most popular and simplest methods used to detect the NO concentration. The Griess assay′s mechanism is summarized as the azo coupling between diazonium species, which are produced from sulfanilamide with NO2, and naphthylethylenediamine. The NO2/NO3 Assay Kit contains these dyes, a nitrate reductase, an enzyme co-factor, buffer solution and NO2, NO3 solutions as standards. Therefore, total NO metabolites are easily detectable using this kit. The suitable NO2 detection range is from 10 to 100 μM.
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Tylko elementy zestawu

Numer produktu
Opis

  • NaNO2 Standard Solution (100 mM) 1 bottle

  • NaNO3 Standard Solution (100 mM) 1 bottle

  • Buffer solution (20 mM, pH 7.6) 1 bottle

  • Nitrate reductase (lyophilized) 1 bottle

  • Enzyme Co-factor (lyophilized) 1 bottle

  • Griess Reagent A 1 bottle

  • Griess Reagent B 1 bottle

produkt powiązany

Numer produktu
Opis
Cennik

Piktogramy

CorrosionExclamation mark

Hasło ostrzegawcze

Warning

Zwroty wskazujące rodzaj zagrożenia

Zwroty wskazujące środki ostrożności

Klasyfikacja zagrożeń

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3

Organy docelowe

Respiratory system

Kod klasy składowania

8A - Combustible corrosive hazardous materials

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

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Odwiedź Bibliotekę dokumentów

Mohamed Raâfet Ben Khedher et al.
Lipids in health and disease, 16(1), 241-241 (2017-12-14)
Vasculogenic erectile dysfunction (VED) is considered as a common complication among people with type 2 diabetes (T2D). We tested whether changes in fatty acid (FAs) classes measured in erythrocytes are associated with increased risk of diabetic VED along with related
Fredrik Sjövall et al.
PloS one, 9(5), e97673-e97673 (2014-05-16)
The levels of nitric oxide (NO) and various cytokines are known to be increased during sepsis. These signaling molecules could potentially act as regulators and underlie the enhancement of mitochondrial function described in the later phase of sepsis. Therefore, we
Wang Kuan-Hong et al.
Archives of medical science : AMS, 14(5), 1112-1118 (2018-08-30)
Redox signaling initiates pathogenesis of neuronal degeneration. Plumbagin is a potential antioxidant with anti-inflammatory, anti-cancer and radio sensitizing properties. In the present study, we aimed to determine the protective role of plumbagin against H2O2-induced neurotoxicity in PC12 cells by determining
Iselin Vildmyren et al.
Nutrients, 10(12) (2018-11-25)
Hypertension is the leading risk factor for cardiovascular disease, and prevention of high blood pressure through diet and lifestyle should be a preferred approach. High intake of fish is associated with lower blood pressure, possibly mediated through the proteins since
Gerlane F De Brito et al.
Meat science, 130, 81-90 (2017-04-26)
The aim of this study was to evaluate the effect of different forage-types on lamb meat quality parameters. White Dorper lambs that had grazed five forage-types, were slaughtered commercially. At 24h post-mortem, the m. longissimus lumborum (LL) was removed from

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