324715

Endoproteinase Lys-C, Sequencing Grade, Lysobacter enzymogenes

• Synonim(y): Endoproteinase Lys-C, Sequencing Grade, Lysobacter enzymogenes
• Numer CAS: 72561-05-8
• Numer EC enzymu: 3.4.21.50 (BRENDA, IUBMB)
• Numer MDL: MFCD02253020
• Kod UNSPSC: 12352202

Właściwości

• klasa czystości: Proteomics Grade
• Poziom jakości: 100
• Próba: ≥90% (SDS-PAGE)
• Postać: lyophilized
• aktywność właściwa: ≥200 units/mg protein
• producent / nazwa handlowa: Calbiochem^®
• warunki przechowywania: OK to freeze; desiccated (hygroscopic)
• Warunki transportu: ambient
• temp. przechowywania: 2-8°C

Opis

Opis ogólny

Native endoproteinase Lys-C from Lysobacter enzymogenes. Serine protease that specifically hydrolyzes amide and peptide ester bonds at the carboxylic side of lysine in peptides and proteins. Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins. Inhibited by aprotinin, DFP, leupeptin, and TLCK.

Native endoproteinase Lys-C from Lysobacter enzymogenes. Serine protease that specifically hydrolyzes amide and peptide ester bonds at the carboxylic side of lysine in peptides and proteins. Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins. Inhibited by aprotinin, DFP, leupeptin, and TLCK. Suggested working concentration: 1:20 to 1:100 (protease:protein by weight) for sequence analysis.

Ostrzeżenie

Toxicity: Harmful (C)

Definicja jednostki

One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol Tos-Gly-Pro-Lys-pNA per min at 25°C, pH 7.7.

Postać fizyczna

Lyophilized from 50 mM HEPES, 10 mM EDTA, 5 mg/ml raffinose, pH 8.0.

Rekonstytucja

Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short-term storage. Stock solutions are stable for up to 2 days at 4°C or for up to 1 month at -20°C.

Reconstitute in 50 µl of distilled H₂O.

Informacje prawne

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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