16-213
Przeciwciało anty-Myc Tag, klon 4A6, koniugat HRP
clone 4A6, Upstate®, from mouse
Synonim(y):
Czynnik transkrypcyjny p64, homolog onkogenu wirusa mielocytomatozy ptaków, białko protoonkogenu myc, homolog onkogenu wirusa mielocytomatozy ptaków v-myc, homolog onkogenu wirusa mielocytomatozy ptaków v-myc (ptasi)
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About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.56
Polecane produkty
pochodzenie biologiczne
mouse
Poziom jakości
białko sprzężone
peroxidase conjugate
forma przeciwciała
purified immunoglobulin
rodzaj przeciwciała
primary antibodies
klon
4A6, monoclonal
reaktywność gatunkowa
human
producent / nazwa handlowa
Upstate®
metody
western blot: suitable
numer dostępu NCBI
numer dostępu UniProt
Warunki transportu
dry ice
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
human ... MYC(4609)
Opis ogólny
Immunogen: peptide corresponding to amino acids 410-420 (MEQKLISEEDL) of human Myc
Specificity: Recognizes and is specific for recombinant proteins containing the Myc epitope tag (EQKLISEEDL) in a variety of sequence contexts. Also recognizes human Myc.
Species Cross-reactivity: Human, others not tested
Ig Type: IgG
Host: Mouse
Molecular Weight: Varies depending upon the protein which is tagged
Specificity: Recognizes and is specific for recombinant proteins containing the Myc epitope tag (EQKLISEEDL) in a variety of sequence contexts. Also recognizes human Myc.
Species Cross-reactivity: Human, others not tested
Ig Type: IgG
Host: Mouse
Molecular Weight: Varies depending upon the protein which is tagged
Myc-Tag is a short synthetic peptide sequence derived from C-terminal region of human c-myc that is used as an epitope tag. The Myc tag can be cloned either upstream or downstream of the target gene, resulting in a fusion protein that allows efficient purification, detection, and localization of target proteins. The relatively small size of this tag vs. other commonly used tags such as GST (30 kDa) makes it less likely to interfere with target protein structure or function. The myc epitope tag is widely used to detect expression of recombinant proteins in bacterial, yeast, insect, and mammalian systems.
Specyficzność
Other species cross-reactivity not tested.
Recognizes and is specific for recombinant proteins containing the Myc epitope tag (EQKLISEEDL) in a variety of sequence contexts. Also recognizes human Myc.
Immunogen
Epitope: C-terminal
KLH-conjugated, synthetic peptide corresponding to amino acids 410-420 (MEQKLISEEDL) of human Myc. Clone 4A6.
Zastosowanie
Anti-Myc Tag Antibody, clone 4A6, HRP conjugate detects level of Myc Tag & has been published & validated for use in WB.
Research Category
Epitope Tags & General Use
Epitope Tags & General Use
Research Sub Category
Epitope Tags
Epitope Tags
Jakość
Evaluated by Western Blot on Myc-PP2A A subunit transfected NIH/3T3 lysates.
Western Blot Analysis:
1:500 dilution of this antibody detected myc-PP2A on 10 µg of Myc-PP2A A subunit transfected NIH/3T3 lysates.
Western Blot Analysis:
1:500 dilution of this antibody detected myc-PP2A on 10 µg of Myc-PP2A A subunit transfected NIH/3T3 lysates.
Opis wartości docelowych
Varies depending upon the protein which is tagged.
Postać fizyczna
Protein G Purified
Purified mouse monoclonal IgG1 conjugated to horseradish peroxidase (HRP) in buffer containing: 50% storage buffer (PBS, 0.05% Kathon®, 0.2 mg/mL BSA) and 50% glycerol.
Przechowywanie i stabilność
Stable for 1 year at -20ºC from date of receipt.
Komentarz do analizy
Control
Positive control for human Myc: Widespread expression including A431 cell lysate, ovarian cancer cell lysate, or breast carcinoma tissue. Myc fusion protein expressed in cells.
Positive control for human Myc: Widespread expression including A431 cell lysate, ovarian cancer cell lysate, or breast carcinoma tissue. Myc fusion protein expressed in cells.
Inne uwagi
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informacje prawne
Kathon is a registered trademark of Rohm and Haas Co.
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Hasło ostrzegawcze
Warning
Zwroty wskazujące rodzaj zagrożenia
Zwroty wskazujące środki ostrożności
Klasyfikacja zagrożeń
Aquatic Chronic 2 - Skin Sens. 1
Kod klasy składowania
10 - Combustible liquids
Klasa zagrożenia wodnego (WGK)
WGK 3
Temperatura zapłonu (°F)
390.2 °F - closed cup - Information refers to the main ingredient.
Temperatura zapłonu (°C)
199 °C - closed cup - Information refers to the main ingredient.
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
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