14-451-M
ROKα/ROCK-II Protein, active, 10 µg
Active, N-terminal His6-tagged recombinant human ROCK-II residues 11-552, for use in Kinase Assays.
Synonim(y):
ROK, Renal carcinoma antigen NY-REN-35, Rho kinase, Rho-associated, coiled-coil-containing protein kinase 1, p160ROCK
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About This Item
Kod UNSPSC:
12352202
eCl@ss:
32160405
NACRES:
NA.41
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pochodzenie biologiczne
human
Poziom jakości
rekombinowane
expressed in baculovirus infected Sf21 cells
Postać
liquid
okres trwałości
6 mo.
masa cząsteczkowa
Mw 63.3 kDa
producent / nazwa handlowa
Upstate®
metody
activity assay: suitable (kinase)
rozpuszczalność
soluble
numer dostępu NCBI
numer dostępu UniProt
temp. przechowywania
−70°C
informacje o genach
human ... ROCK1(6093)
Opis ogólny
Research area: APOPTOSIS
N-terminal His6-tagged recombinant human ROCK-II residues 11-552
Product Source: expressed by baculovirus in Sf21 insect cells
Rho kinase (ROCK) is a type of serine/threonine kinase. There are two isoforms, ROCK1 and ROCK2. In humans ROCK1 and ROCK2 are composed of 33 exons and located on chromosome 18 (18q11.1). The ROCK protein structure is characterized by a catalytic kinase domain and Rho-binding domain (RBD) at N-terminal and pleckstrin-homology (PH) domain and an internal cysteine-rich (CR) domain at C-terminal.
N-terminal His6-tagged recombinant human ROCK-II residues 11-552
Product Source: expressed by baculovirus in Sf21 insect cells
Rho kinase (ROCK) is a type of serine/threonine kinase. There are two isoforms, ROCK1 and ROCK2. In humans ROCK1 and ROCK2 are composed of 33 exons and located on chromosome 18 (18q11.1). The ROCK protein structure is characterized by a catalytic kinase domain and Rho-binding domain (RBD) at N-terminal and pleckstrin-homology (PH) domain and an internal cysteine-rich (CR) domain at C-terminal.
Działania biochem./fizjol.
Protein Target: ROKα/ROCK-II
Target Sub-Family: AGC
ROCK (Rho kinase) is activated by interacting with Rho GTPase enzyme. ROCK plays a crucial role in various essential cellular functions like adhesion, contraction, migration, and proliferation. Moreover, it regulates the cytoskeleton and actomyosin contractility during apoptosis. Overexpression of ROCK protein has been observed in various types of cancer such as osteosarcoma, hepatocellular carcinomas, colon and bladder cancers.
Target Sub-Family: AGC
ROCK (Rho kinase) is activated by interacting with Rho GTPase enzyme. ROCK plays a crucial role in various essential cellular functions like adhesion, contraction, migration, and proliferation. Moreover, it regulates the cytoskeleton and actomyosin contractility during apoptosis. Overexpression of ROCK protein has been observed in various types of cancer such as osteosarcoma, hepatocellular carcinomas, colon and bladder cancers.
Opakowanie
Also available in 250μg size (14-451M), please inquire for pricing and availability.
Jakość
routinely evaluated by phosphorylation of the Long S6 Kinase substrate peptide
Postać fizyczna
Ni2+/NTA-agarose
Przechowywanie i stabilność
6 months at -70°C
Inne uwagi
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Informacje prawne
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
J Feng et al.
The Journal of biological chemistry, 274(6), 3744-3752 (1999-01-28)
Rho-associated kinase (Rho-kinase) from chicken gizzard smooth muscle was purified to apparent homogeneity (160 kDa on SDS-polyacrylamide gel electrophoresis) and identified as the ROKalpha isoform. Several substrates were phosphorylated. Rates with myosin phosphatase target subunit 1 (MYPT1), myosin, and the
E Sahai et al.
Current biology : CB, 9(3), 136-145 (1999-02-18)
The Ras-related GTPase RhoA controls signalling processes required for cytoskeletal reorganisation, transcriptional regulation, and transformation. The ability of RhoA mutants to transform cells correlates not with transcription but with their ability to bind ROCK-I, an effector kinase involved in cytoskeletal
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