1.25061

Fractogel^® EMD SO₃ ⁻ (M) Resin

MiniChrom Column Fractogel^® SO3 (M), volume 1 mL, prepacked column

• Synonim(y): MiniChrom Column Fractogel^® SO3 (M), 1 ml
• Kod UNSPSC: 41115711

Właściwości

• ligand: (Sulfoisobutyl)
• Poziom jakości: 100
• Materiały: polypropylene housing
• linia produktu: Fractogel^®
• Postać: prepacked column
• Parametry: ≤8 bar pressure; ~200 cm/hr max. flow rate
• column I.D. × L: 8 mm × 20 mm
• pojemność: 1 mL ; ~130 mL, resin binding capacity
• grupa aktywna macierzy: Methacrylate
• średnia wielkość cząstek: 40-90 μm
• Zastosowanie: mAb; recombinant protein; vaccine development
• metoda separacji: cation exchange
• temp. przechowywania: 2-30°C

Opis

Opis ogólny

8 x 20mm

Cechy i korzyści

MiniChrom Column Fractogel^® SO3 (M), 1 ml enables:

• Increased binding strength at low pH
• High aggregate removal efficiency combined with high dynamic binding capacity
• Lower elution volumes for the highest purity levels
• Compatibility with 2.5 % (v/v) aqueous benzyl alcohol containing 150 mM NaCl storage solution

Due to the titration behavior, the ion exchange capacity can be used from pH 2 to pH 12. The separation of proteins is based on reversible electrostatic interactions between the surface′s positively charged regions and the support. Proteins are retained efficiently on Fractogel^® EMD SO₃^- (M) when the pH of the buffer is about 1 unit below their isoelectric points (pl).

The strength of the binding depends on the following:

• the buffer system
• pH value of the buffer which determines the surface charge of the protein
• the concentration of the counter ions
• the charge density on the support (protein binding capacity)

Komentarz do analizy

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Informacje prawne

FRACTOGEL is a registered trademark of Merck KGaA, Darmstadt, Germany

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