06-284-AF647
Anti-Nitrotyrosine Antibody Alexa Fluor™ 647 Conjugate
from rabbit, ALEXA FLUOR™ 647
Synonim(y):
Nitrotyrosine AlexaFluor™ 647 Conjugate
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About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.45
Polecane produkty
pochodzenie biologiczne
rabbit
Poziom jakości
białko sprzężone
ALEXA FLUOR™ 647
forma przeciwciała
affinity isolated antibody
rodzaj przeciwciała
primary antibodies
klon
polyclonal
reaktywność gatunkowa
human
reaktywność gatunkowa (przewidywana na podstawie homologii)
all
metody
immunocytochemistry: suitable
Warunki transportu
wet ice
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
human ... NOS1(4842)
Opis ogólny
Nitrotyrosine is produced by the action of reactive nitrogen species, such as peroxinitrite and nitrogen dioxide on tyrosine residues in proteins. Nitrotyrosine is an indicator of cell damage, inflammation, and nitric oxide production. Both free tyrosine and protein bound tyrosine residues are prone to nitration.
In several pathological conditions, oxidative stress is increased resulting in the formation of super oxide and nitric oxide that react to form peroxynitrite (ONOO-), which is a highly reactive molecule. It causes oxidation of proteins and lipids and DNA damage, which results in loss of protein and lipid function and can increase the probability of gene mutations. Higher incidence of protein nitration has been reported in diabetes, atherosclerotic plaques, and rheumatoid arthritis, among others. Nitrotyrosine can be measured by amino-acid analysis of protein hydrolysates and detected cells and tissues by immunocytochemical techniques using antibodies developed against the nitrotyrosine.
In several pathological conditions, oxidative stress is increased resulting in the formation of super oxide and nitric oxide that react to form peroxynitrite (ONOO-), which is a highly reactive molecule. It causes oxidation of proteins and lipids and DNA damage, which results in loss of protein and lipid function and can increase the probability of gene mutations. Higher incidence of protein nitration has been reported in diabetes, atherosclerotic plaques, and rheumatoid arthritis, among others. Nitrotyrosine can be measured by amino-acid analysis of protein hydrolysates and detected cells and tissues by immunocytochemical techniques using antibodies developed against the nitrotyrosine.
Specyficzność
This rabbit polyclonal antibody detects tyrosine nitrated proteins.
Immunogen
Epitope: Nitrated Tyrosine
Nitrated Keyhole Limpet hemocyanain (KLH)
Zastosowanie
Anti-Nitrotyrosine AlexaFluor 647 Conjugate, Cat. No. 06-284-AF647, is a rabbit polyclonal antibody that detects tyrosine nitrated proteins and has been tested for use in Immunocytochemistry.
Research Category
Signaling
Signaling
Research Sub Category
Developmental Signaling
Developmental Signaling
The unconjugated antibody (Cat. No. 06-284) is shown to be suitable also for Western blotting, immunocytochemistry, immunohistochemistry, and immunoprecipitation.
Jakość
Evaluated by Immunocytochemistry in A431 treated with peroxytnitrite.
Immunocytochemistry Analysis: 1:100 dilution of this antibody detected Nitrotyrosine in A431 cells treated with 5 mM peroxytnitrite.
Immunocytochemistry Analysis: 1:100 dilution of this antibody detected Nitrotyrosine in A431 cells treated with 5 mM peroxytnitrite.
Postać fizyczna
Affinity purified
Purified rabbit polyclonal antibody in buffer containing PBS, pH7.1, and 15 mg/mL BSA with 0.1% sodium azide.
Przechowywanie i stabilność
Stable for 1 year at 2-8°C from date of receipt.
Inne uwagi
Concentration: Please refer to lot specific datasheet.
Informacje prawne
ALEXA FLUOR is a trademark of Life Technologies
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania
12 - Non Combustible Liquids
Klasa zagrożenia wodnego (WGK)
WGK 2
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
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