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Key Documents

05-173

Sigma-Aldrich

Anti-Calmodulin Antibody

Upstate®, from mouse

Synonim(y):

calmodulin 1, calmodulin 1 (phosphorylase kinase, delta), phosphorylase kinase, delta subunit

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About This Item

Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

pochodzenie biologiczne

mouse

Poziom jakości

forma przeciwciała

purified antibody

rodzaj przeciwciała

primary antibodies

klon

monoclonal

reaktywność gatunkowa

pig, mouse, vertebrates, rat, human, bovine

producent / nazwa handlowa

Upstate®

metody

immunoprecipitation (IP): suitable
western blot: suitable

izotyp

IgG1

numer dostępu NCBI

numer dostępu UniProt

Warunki transportu

dry ice

docelowa modyfikacja potranslacyjna

unmodified

informacje o genach

human ... CALM3(808)

Opis ogólny

Calmodulin (CaM) is a ubiquitous, calcium-binding protein that can bind to and regulate a multitude of different protein targets, thereby affecting many different cellular functions.
CaM mediates processes such as inflammation, metabolism, apoptosis, muscle contraction, intracellular movement, short-term and long-term memory, nerve growth and the immune response. CaM is expressed in many cell types and can have different subcellular locations, including the cytoplasm, within organelles, or associated with the plasma or organelle membranes. Many of the proteins that CaM binds are unable to bind calcium themselves, and as such use CaM as a calcium sensor and signal transducer. CaM undergoes a conformational change upon binding to calcium, which enables it to bind to specific proteins for a specific response. CaM can bind up to four calcium ions, and can undergo post-translational modifications, such as phosphorylation, acetylation, methylation and proteolytic cleavage, each of which can potentially modulate its actions.

Specyficzność

Also reacts to wheat germ. Wide cross-reactivity expected.
Specific for both Ca2+-bound and Ca2+-free calmodulin, Mr 17 kDa. Does not recognize bovine S100-alpha, S100-beta, troponin C or parvalbumin.

Immunogen

Epitope: a.a. 128-148
Synthetic peptide corresponding to the 21 carboxy-terminal amino acids (128-148) of bovine calmodulin, conjugated with tyroglobulin.

Zastosowanie

Detect Calmodulin using this Anti-Calmodulin Antibody validated for use in WB & IP.
Immunoprecipitation:
4 μg of a previous lot immunoprecipitated calmodulin from A431 RIPA cell lysate. Radioimmunoassay:
Use 10 μg/mL to detect calmodulin in the range of 200-300 ng.
Research Category
Signaling
Research Sub Category
GPCR, cAMP/cGMP & Calcium Signaling

Jakość

Evaluated on human A431 carcinoma cells, RIPA lysates from mouse 3T3 and L6 rat skeletal fibroblasts.

Western Blot Analysis: 0.1-1 μg/mL of this antibody detected calmodulin in RIPA lysates from human A431 carcinoma cells. Previous lots detected calmodulin in RIPA lysates from mouse 3T3 and L6 rat skeletal fibroblasts.

Opis wartości docelowych

17 kDa

Powiązanie

Replaces: 04-1158

Postać fizyczna

Format: Purified
Protein G Chromatography
Purified mouse IgG1 in storage buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide. Frozen solution.

Przechowywanie i stabilność

Stable for 1 year at from date of receipt.

Komentarz do analizy

Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.

Inne uwagi

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informacje prawne

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania

10 - Combustible liquids

Klasa zagrożenia wodnego (WGK)

WGK 1


Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Translocation of calmodulin to the nucleus supports CREB phosphorylation in hippocampal neurons.
Deisseroth, K, et al.
Nature, 392, 198-202 (1998)
A Lee et al.
Nature, 399(6732), 155-159 (1999-05-21)
Neurotransmitter release at many central synapses is initiated by an influx of calcium ions through P/Q-type calcium channels, which are densely localized in nerve terminals. Because neurotransmitter release is proportional to the fourth power of calcium concentration, regulation of its
Human platelets contain estrogen receptor alpha, caveolin-1 and estrogen receptor associated proteins.
Muthuvel Jayachandran, Virginia M Miller, Muthuvel Jayachandran, Virginia M Miller
Platelets null
Calmodulin regulates L-selectin adhesion molecule expression and function through a protease-dependent mechanism
Kahn, J., et al
Cell, 92, 809-818 (1998)
Induction of TLR4-target genes entails calcium/calmodulin-dependent regulation of chromatin remodeling.
Lai, D; Wan, M; Wu, J; Preston-Hurlburt, P; Kushwaha, R; Grundstrom, T; Imbalzano, AN; Chi, T
Proceedings of the National Academy of Sciences of the USA null

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