05-1355
Anti-acetyl Histone H4 (Lys5/8/12/16) Antibody, clone 3HH4-4C10
ascites fluid, clone 3HH4-4C10, from mouse
Synonim(y):
H4KAc, Histone H4 (acetyl), H4 histone family, member N, H4 histone, family 2, histone 2, H4, histone 2, H4a, histone IV, family 2, histone cluster 2, H4a
Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych
About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
klon:
3HH4-4C10, monoclonal
application:
ICC
WB
WB
reaktywność gatunkowa:
human
metody:
immunocytochemistry: suitable
western blot: suitable
western blot: suitable
citations:
12
Polecane produkty
pochodzenie biologiczne
mouse
Poziom jakości
forma przeciwciała
ascites fluid
rodzaj przeciwciała
primary antibodies
klon
3HH4-4C10, monoclonal
reaktywność gatunkowa
human
reaktywność gatunkowa (przewidywana na podstawie homologii)
mouse (based on 100% sequence homology), rat (based on 100% sequence homology)
metody
immunocytochemistry: suitable
western blot: suitable
izotyp
IgG1κ
numer dostępu NCBI
numer dostępu UniProt
Warunki transportu
dry ice
docelowa modyfikacja potranslacyjna
acetylation (Lys5/Lys8/Lys12/Lys16)
informacje o genach
human ... H4C1(8359)
mouse ... H4C1(326619)
Opis ogólny
Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. Acetylation of histone H4 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs). Posttranslational acetylation of the conserved core histone N-terminal tail domains is typically associated with gene activation.
Specyficzność
This antibody recognizes Histone H4.
Immunogen
Epitope: Lys5/8/12/16
Linear peptide corresponding to human Histone H4 acetylated at Lys5/8/12/16.
Zastosowanie
Immunocytochemistry Analysis:
1:500 dilution from a representative lot detected Histone H4 in A431 and HeLa cells.
1:500 dilution from a representative lot detected Histone H4 in A431 and HeLa cells.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
Use Anti-acetyl Histone H4 (Lys5/8/12/16) Antibody, clone 3HH4-4C10 (mouse monoclonal antibody) validated in WB, ICC to detect acetyl Histone H4 (Lys5/8/12/16) also known as H4KAc, Histone H4 (acetyl).
Jakość
Evaluated by Western Blot in HeLa acid extract lysate.
Western Blot Analysis: 1:2,000 dilution of this antibody detected Histone H4 on 10 µg of HeLa acid extract lysate.
Western Blot Analysis: 1:2,000 dilution of this antibody detected Histone H4 on 10 µg of HeLa acid extract lysate.
Opis wartości docelowych
~ 13 kDa
Postać fizyczna
Unpurified
Unpurified mouse monoclonal IgG1κ preservative-free ascites.
Przechowywanie i stabilność
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Komentarz do analizy
Control
HeLa acid extract lysate
HeLa acid extract lysate
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania
12 - Non Combustible Liquids
Klasa zagrożenia wodnego (WGK)
WGK 1
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
Masz już ten produkt?
Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Jingwen Feng et al.
Scientific reports, 7(1), 9581-9581 (2017-08-31)
High-content screening is commonly used in studies of the DNA damage response. The double-strand break (DSB) is one of the most harmful types of DNA damage lesions. The conventional method used to quantify DSBs is γH2AX foci counting, which requires
Yolanda Colino-Sanguino et al.
iScience, 21, 773-788 (2019-11-16)
Acetylation of the histone variant H2A.Z (H2A.Zac) occurs at active regulatory regions associated with gene expression. Although the Tip60 complex is proposed to acetylate H2A.Z, functional studies suggest additional enzymes are involved. Here, we show that p300 acetylates H2A.Z at
Maxime Wery et al.
PLoS genetics, 14(7), e1007465-e1007465 (2018-07-06)
Antisense (as)lncRNAs can regulate gene expression but the underlying mechanisms and the different cofactors involved remain unclear. Using Native Elongating Transcript sequencing, here we show that stabilization of antisense Exo2-sensitivite lncRNAs (XUTs) results in the attenuation, at the nascent transcription
Teresa Chioccarelli et al.
International journal of molecular sciences, 21(9) (2020-05-02)
The cannabinoid receptor CB1 regulates differentiation of spermatids. We recently characterized spermatozoa from caput epididymis of CB1-knock-out mice and identified a considerable number of sperm cells with chromatin abnormality such as elevated histone content and poorly condensed chromatin. In this
Jessica M Bryant et al.
Molecular and cellular biology, 35(8), 1433-1448 (2015-02-19)
During spermiogenesis, the postmeiotic phase of mammalian spermatogenesis, transcription is progressively repressed as nuclei of haploid spermatids are compacted through a dramatic chromatin reorganization involving hyperacetylation and replacement of most histones with protamines. Although BRDT functions in transcription and histone
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