04-1488
Anti-HIRA Antibody, clone WC119
clone WC119, from mouse
Synonim(y):
DiGeorge critical region gene 1, HIR (histone cell cycle regulation defective) homolog A (S. cerevisiae), HIR histone cell cycle regulation defective homolog A, HIR histone cell cycle regulation defective homolog A (S. cerevisiae), TUP1-like enhancer of
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About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Polecane produkty
pochodzenie biologiczne
mouse
Poziom jakości
forma przeciwciała
purified immunoglobulin
rodzaj przeciwciała
primary antibodies
klon
WC119, monoclonal
reaktywność gatunkowa
mouse
reaktywność gatunkowa (przewidywana na podstawie homologii)
human (based on 100% sequence homology)
metody
immunoprecipitation (IP): suitable
western blot: suitable
izotyp
IgG1κ
numer dostępu NCBI
numer dostępu UniProt
Warunki transportu
wet ice
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
human ... HIRA(7290)
Opis ogólny
HIRA (TUP1 like enhancer of split protein 1) is a protein localized primarily in the nucleus. It functions as a histone chaparone to preferentially place histone H3.3 on nucleosome. It is broadly expressed in a variety of tissues and is expressed during embryogenesis. Data suggests HIRA plays a part in mechanisms of transcriptional regulation similar to that played by yeast HIR1 and HIR2 together. The HIRA protein has been shown to interact with HIRIP3, HIRIP5, histones H2B and H4, and PAX3. HIRA may play a part in the etiology of the DiGeorge syndrome (DGS), a developmental disorder. The clinical features of this disease include absence or hypoplasia of the thymus and parathyroid glands, cardiovascular malformations, facial dysplasia, a cleft palate and mental retardation.
Specyficzność
This antibody recognizes HIRA.
Immunogen
Epitope: Unknown
GST-tagged recombinant protein corresponding to human HIRA.
Zastosowanie
Anti-HIRA Antibody, clone WC119 is a Mouse Monoclonal Antibody for detection of HIRA also known as HIR (histone cell cycle regulation defective) homolog & has been validated in WB & IP.
Immunoprecipitation Analysis: A previous lot was used by an independent laboratory in IP. (Hall, C., et al. (2001). Molecular and Cellular Biology. 21(5):1854-1865.)
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology
Chaperones
Chromatin Biology
Chaperones
Jakość
Evaluated by Western Blot in NIH/3T3 cell lysate.
Western Blot Analysis: 0.44 µg/ml of this antibody detected HIRA on 10 µg of NIH/3T3 cell lysate.
Western Blot Analysis: 0.44 µg/ml of this antibody detected HIRA on 10 µg of NIH/3T3 cell lysate.
Opis wartości docelowych
~ 112 kDa
Postać fizyczna
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Przechowywanie i stabilność
Stable for 1 year at 2-8°C from date of receipt.
Komentarz do analizy
Control
NIH/3T3 cell lysate
NIH/3T3 cell lysate
Inne uwagi
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania
12 - Non Combustible Liquids
Klasa zagrożenia wodnego (WGK)
WGK 1
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
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