04-1481

Anti-RPA2 p34 Antibody, clone RPA20 1-46

from mouse, clone RPA20, clone 1-46

• Synonim(y): RF-A protein 2, RP-A p32, RP-A p34, Replication factor A protein 2, replication protein A2 (32kD), replication protein A2, 32kDa, Replication protein A 32kDa subunit, RF-A, replication factor-A protein 2, p32, p34, RPA2, REPA2, RPA32.
• Kod UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: mouse
• Poziom jakości: 100
• forma przeciwciała: affinity isolated antibody
• rodzaj przeciwciała: primary antibodies
• klon: 1-46, monoclonal; RPA20, monoclonal
• reaktywność gatunkowa: human, mouse
• opakowanie: antibody small pack of 25 μg
• metody: western blot: suitable
• izotyp: IgG1κ
• numer dostępu NCBI: NM_002946
• numer dostępu UniProt: P15927
• Warunki transportu: ambient
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: human ... RPA2(6118)

Opis

Opis ogólny

RPA is a heterotrimeric protein complex that binds specifically to single-stranded DNA (ssDNA). It is composed of three subunits, RPA1 (70 kDa), RPA2 (32 kDa), and RPA3 (14 kDa), and plays multiple roles in DNA metabolism. RPA is required for DNA replication initiation, as well as replication elongation. At the onset of DNA replication, RPA is loaded onto chromatin after the binding of Cdc45 to origins. RPA is needed for subsequent loading of DNA polymerase and other replication proteins to initiate DNA replication. After DNA replication begins, RPA moves with replication forks, stabilizing ssDNA and assisting in DNA synthesis. In addition to its replication function, RPA is also known to play essential roles in damage repair and recombination.The 32 kDa subunit, is phosphorylated by the cdc2 family of kinases when cells enter S-phase and in response to DNA damage by ATM, ATR, and DNA-PK. Alternate names for RPA32 include replication protein A 32 kDa subunit, RP-A, RF-A, replication factor-A protein 2, p32, p34, RPA2, REPA2, and RPA32.

Specyficzność

This antibody recognizes RPA2.

Immunogen

Epitope: Unknown

His-tagged recombinant protein corresponding to human RPA2.

Zastosowanie

Anti-RPA2 p34 Antibody, clone RPA20 1-46 is a Mouse Monoclonal Antibody for detection of RPA2 p34 also known as Replication factor A protein 2 & has been validated in WB.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

Chromatin Biology

Western Blot (SNAP ID) Analysis: 0.5 µg/mL from a previous lot detected RPA2 on 10 µg of NIH/3T3 cell lysate.

Jakość

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected RPA2 on 10 µg of HeLa cell lysate.

Opis wartości docelowych

~32 kDa

Postać fizyczna

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Przechowywanie i stabilność

Stable for 1 year at 2-8°C from date of receipt.

Komentarz do analizy

Control
HeLa cell lysate

Inne uwagi

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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