추천 제품
생물학적 소스
microbial
Quality Level
양식
lyophilized powder
특이 활성도
≥7 units/mg solid
분자량
~160 kDa
기술
inhibition assay: suitable
색상
brown
최적 pH
7.5-8.0
배송 상태
wet ice
저장 온도
−20°C
유전자 정보
Escherichia coli K12 ... xdhA(947116) , xdhB(947205) , xdhC(945148)
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일반 설명
Research area: Cell Cycle
Xanthine Oxidase (XO), a versatile molybdoflavoprotein, is one of the forms of xanthine oxidoreductases (XORs). XO is characterized with two flavin molecules (FAD), two molybdenum atoms, and eight iron atoms bound per enzymatic unit.
Xanthine Oxidase (XO), a versatile molybdoflavoprotein, is one of the forms of xanthine oxidoreductases (XORs). XO is characterized with two flavin molecules (FAD), two molybdenum atoms, and eight iron atoms bound per enzymatic unit.
애플리케이션
Xanthine Oxidase microbial has been used:
- to determine in vitro xanthine oxidase activity
- as a source to generate superoxide anions during superoxide dismutase assay
- as a component of the reagent mix in colorimetric paper-based device to detect allopurinol in traditional medicine by enzymatic reaction
생화학적/생리학적 작용
Xanthine Oxidase catalyzes the hydroxylation of hypoxanthine to xanthine and xanthine to uric acid and oxygen free radicals during purine metabolism. This enzyme is also implicated in caffeine metabolism. XO also plays a vital role in the catabolism of xenobiotics including various drugs such as thiopurine drugs, containing 6-mercaptopurine, allopurinol, and uric acid. Excess of XO activity causes hyperuricemia (higher levels of uric acid in the blood), which further leads to gout.
Also contains proteolytic activity.
물리적 특성
Inhibitors: Reducing agents, Hg++, Ag+,MIA
Optimum pH: 7.5 - 8.0
Optimum temperature: 65oC
pH Stability: pH 6.5 - 9.0 (25oC, 15hr)
Thermal stability: below 55oC (pH 8.0, 30min)
Optimum pH: 7.5 - 8.0
Optimum temperature: 65oC
pH Stability: pH 6.5 - 9.0 (25oC, 15hr)
Thermal stability: below 55oC (pH 8.0, 30min)
단위 정의
One unit will convert 1.0 μmole of xanthine to uric acid per min at pH 7.5 at 25 °C. Approx. 50% of the activity is obtained with hypoxanthine as substrate.
물리적 형태
Lyophilized powder containing BSA and sodium glutamate as stabilizers
저장 및 안정성
Tightly closed. Dry. Keep locked up or in an area accessible only to qualified or authorized
persons
persons
저해제
제품 번호
설명
가격
신호어
Danger
유해 및 위험 성명서
Hazard Classifications
Resp. Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
개인 보호 장비
Eyeshields, Gloves, type N95 (US)
이미 열람한 고객
Pál Pacher et al.
Pharmacological reviews, 58(1), 87-114 (2006-03-02)
The prototypical xanthine oxidase (XO) inhibitor allopurinol, has been the cornerstone of the clinical management of gout and conditions associated with hyperuricemia for several decades. More recent data indicate that XO also plays an important role in various forms of
Michael C Hall
Molecular and cellular biochemistry, 292(1-2), 1-11 (2006-09-20)
The cytotoxicity of five oxysterols identified in cooked fish, 7-ketocholesterol, 7beta-hydroxycholesterol, cholesterol 5alpha,6alpha-epoxide, cholestanetriol and 4-cholesten-3-one, was investigated in primary cultures of bovine ovarian granulosa cells. Cells were exposed to the oxysterols individually and to a mixture of the same
P J Marro et al.
Pediatric research, 41(4 Pt 1), 513-520 (1997-04-01)
The effect of allopurinol to inhibit purine metabolism via the xanthine oxidase pathway in neonates with severe, progressive hypoxemia during rescue and reperfusion with extracorporeal membrane oxygenation (ECMO) was examined. Twenty-five term infants meeting ECMO criteria were randomized in a
Kavita R Hegde et al.
Ophthalmologica. Journal international d'ophtalmologie. International journal of ophthalmology. Zeitschrift fur Augenheilkunde, 222(3), 194-198 (2008-05-24)
Oxidative stress is considered to be a significant risk factor in the pathogenesis of many eye diseases associated with aging. The purpose of this study was to investigate the susceptibility of retinal tissue to reactive oxygen species (ROS)-induced damage, and
Zafer Teke et al.
Journal of investigative surgery : the official journal of the Academy of Surgical Research, 25(6), 354-365 (2012-12-12)
We aimed to investigate the effects of caffeic acid phenethyl ester (CAPE) on intestinal mucosal injury induced by superior mesenteric occlusion. This experimental study was conducted on 48 male Wistar-albino rats. The animals were randomly allocated into four groups: (i)
프로토콜
The appearance of uric acid is measured at 293nm by spectrophotometry.
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