X1875

Xanthine Oxidase from bovine milk

Grade I, ammonium sulfate suspension, ≥0.4 units/mg protein

• 동의어(들): XOD, Xanthine:oxygen oxidoreductase
• CAS Number: 9002-17-9
• 효소 위원회 번호: 1.17.3.2.
• EC Number: 232-657-6
• MDL number: MFCD00082145
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 유형: Grade I
• Quality Level: 200
• 양식: ammonium sulfate suspension
• 특이 활성도: ≥0.4 units/mg protein
• 외래 활성: uricase ≤0.5%
• 배송 상태: wet ice
• 저장 온도: 2-8°C

설명

일반 설명

Formerly E.C. 1.1.3.22

Xanthine Oxidase (XOD) is a metal flavoprotein. It has flavin adenine dinucleotide (FAD), molybdenum and iron in the ratio 2:2:8. This homodimer has a molecule weight of 290kDa. It is a member of the molybdenum-protein family. This enzyme consists of two separated substrate-binding sites.

애플리케이션

Xanthine Oxidase from bovine milk has been used:

• in the preparation of xanthine oxidase (XO) solution for 5-(diethoxyphosphoryl)-5-methyl-1-pyrroline-N-oxide (DEPMPO)-spin trapping assay
• in in vitro XO assay for screening Vietnamese medicinal plants for XO inhibitory activity
• as a standard to determine XO activity
• as a standard to test the synergistic effect of docosahexaenoic acid (DHA)

생화학적/생리학적 작용

Hydroxylation of hypoxanthine to xanthine and xanthine to uric acid is catalyzed by xanthinebb oxidase (XO) enzyme.

Xanthine oxidase is a molybdenum-containing enzyme that is found in the cytosol, and may be strongly inhibited by flavonoids. It plays a vital role in the metabolism of some drugs, as well as purines and pyrimidines. It is also known to be a biological source of reactive oxygen species.

Xanthine oxidase was shown to be involved in the reduction of cytochrome c by the generation of superoxide anions following the oxidation of xanthine. These free radicals are responsible for reducing cytochrome c.

단위 정의

One unit will convert 1.0 μmole of xanthine to uric acid per min at pH 7.5 at 25 °C. Approx. 50% of the activity is obtained with hypoxanthine as substrate.

물리적 형태

Suspension in 2.3 M (NH₄)₂SO₄ containing 1 mM sodium salicylate

분석 메모

Protein determined by biuret

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H334
• 예방조치 성명서: P261 - P284 - P304 + P340 + P312 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)