N1665

5′-Nucleotidase human

recombinant, expressed in CHO cells, vial of 6-12 μg

• 동의어(들): CD73, NT5E, ecto-5′-nucleotidase
• CAS Number: 9027-73-0
• 효소 위원회 번호: 3.1.3.5 (BRENDA, IUBMB)
• MDL number: MFCD00130311
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 재조합: expressed in CHO cells
• Quality Level: 200
• 설명: contains a C-terminal 6-His tag
• 분석: ≥90% (SDS-PAGE)
• 형태: solution
• enzyme activity: ≥15 units/mg protein
• 분자량: ~61 kDa by SDS-PAGE (reducing)
• 포장: vial of 6-12 μg
• 불순물: ≤1.0 EU/μg endotoxin
• NCBI 수납 번호: NP_002517
• 배송 상태: dry ice
• 저장 온도: −70°C
• 유전자 정보: human ... NT5E(4907)

설명

일반 설명

5′-Nucleotidase is an extracellular enzyme found in a wide variety of mammalian cells. It helps to accelerate the hydrolysis reaction of phosphate group from 5′ nucleotides, resulting in corresponding nucleosides.

생화학적/생리학적 작용

5′-Nucleotidase has various clinical significances. It is a key molecule in the regulation of cancer cells proliferation, migration and invasion in vitro tumor angiogenesis, and tumor immune escape in vivo. Due to this important role, the enzyme is a potential target for cancer research. It is also involved in salvage of extracellular nucleotides and plays a key role in the control of tissue homeostasis.

5′-nucleotidase, also known as CD73, is an extracellular enzyme that converts nucleoside-5′−monophosphates to nucleosides with a substrate preference of AMP. Native 5′-nucleotidase is a GPI-anchored protein whose exporession is upregulated by hypoxia. 5′-nucleotidase has many functions in vivo including the generation of extracellular adenosine.

단위 정의

One unit will hydrolyze 1.0 μmole of inorganic phosphorus from adenosine 5′-monophosphate per min at pH 9.0 at 37 °C.

물리적 형태

Supplied as a solution containing Tris, NaCl, CaCl_2, and 20% glycerol.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable