추천 제품
양식
powder
특이 활성도
≥1,500 NIH units/mg protein (E1%/280 = 19.5)
분자량
heavy chain ~31 kDa
light chain ~6 kDa
기술
cell culture | mammalian: suitable
UniProt 수납 번호
배송 상태
dry ice
저장 온도
−20°C
유전자 정보
cow ... F2(280685)
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일반 설명
Thrombin is the final coagulation protease in regard to hemostasis, promoting both procoagulant and anticoagulant effects.
애플리케이션
Thrombin is used for site specific cleavage of recombinant fusion proteins containing an accessible thrombin recognition site for removal of affinity tags. Thrombin has been used in a study to assess an expression and purification system for the biosynthesis of adenosine receptor peptides for biophysical and structural characterization.
생화학적/생리학적 작용
Serine protease that selectively cleaves Arg-Gly bonds in fibrinogen to form fibrin and fibrinopeptides A and B.
단위 정의
Activity is expressed in NIH units, and is measured by direct comparison to an NIH thrombin reference standard.
재구성
When reconstituted with 1 mL water, vial contains stated activity in 0.15 M sodium chloride and 0.05 M sodium citrate, pH 6.5.
분석 메모
Activity is expressed in NIH units obtained by direct comparison to a NIH Thrombin Reference Standard, Lot K.
The NIH assay procedure uses 0.2 mL of diluted plasma (1:1 with saline) as a substrate and 0.1 mL of thrombin sample (stabilized in a 1% buffered albumin solution) based on a modification of the method of Biggs. Only clotting times in the range of 15-25 seconds are used for determining thrombin concentrations.
기타 정보
View more information on thrombin at www.sigma-aldrich.com/enzymeexplorer.
기질
저해제
제품 번호
설명
가격
신호어
Danger
유해 및 위험 성명서
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
표적 기관
Respiratory system
Storage Class Code
11 - Combustible Solids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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