D0879

Diisopropylfluorophosphate

• 동의어(들): DFP, DIFP, Diisopropyl phosphorofluoridate, Phosphoric acid diisopropyl ester fluoride
• Linear Formula: [(CH₃)₂CHO]₂POF
• CAS Number: 55-91-4
• Molecular Weight: 184.15
• Beilstein: 1723307
• EC Number: 200-247-6
• MDL number: MFCD00008873
• UNSPSC 코드: 12352200
• PubChem Substance ID: 24893303
• NACRES: NA.77

속성

• 생물학적 소스: synthetic (organic)
• Quality Level: 100
• vapor pressure: 0.58 mmHg ( 20 °C)
• 양식: liquid
• refractive index: n20/D 1.385 (lit.)
• bp: 62 °C/9 mmHg (lit.)
• mp: −82 °C (lit.)
• solubility: anhydrous isopropanol: 0.1-0.5 M (stable for months if stored at -70 °C); H₂O: 1.5% at 25 °C (very unstable (at pH 7.5, half-life = 1 hr); decomposed by alkali.)
• density: 1.06 g/mL at 25 °C (lit.)
• 항생제 활성 스펙트럼: Gram-negative bacteria; Gram-positive bacteria
• 동작 모드: protein synthesis | interferes
• 저장 온도: 2-8°C
• SMILES string: CC(C)OP(F)(=O)OC(C)C
• InChI: 1S/C6H14FO3P/c1-5(2)9-11(7,8)10-6(3)4/h5-6H,1-4H3
• InChI key: MUCZHBLJLSDCSD-UHFFFAOYSA-N
• 유전자 정보: human ... ACHE(43) , PTGS1(5742) , PTGS2(5743)

설명

애플리케이션

Diisopropylfluorophosphate has been used:

• in combination with inactivated thrombin in Affi-Gel 10 column for the purification of recombinant human thrombomodulin with its chondroitin sulfate chain (shrTMCSA)
• for inducing status epilepticus (SE) in adult rats
• as an irreversible serine protease inhibitor in human neutrophil subcellular fractions

생화학적/생리학적 작용

Potent inhibitor of serine proteases and acetylcholinesterase; inhibits cathepsin G, cholinesterase, coagulation factor Xa, leucocyte elastase, pancreatic elastase, tissue kallikrein, plasmin, subtilisin, and thrombin. Inhibits apoptosis induced by ricin and bacterial toxins.

Potent inhibitor of serine proteases such as trypsin and chymotrypsin, and of acetylcholinesterase; also inhibits cathepsin G, cholinesterase, coagulation factor Xa, leucocyte elastase, pancreatic elastase, tissue kallikrein, plasmin, subtilisin, and thrombin. Inhibition of acetylcholinesterase makes this compound especially toxic. Inhibits apoptosis induced by ricin and bacterial toxins.

분석 메모

Typically used at a concentration of 0.10 mM. A safer alternative inhibitor for serine protease is phenylmethylsulfonyl fluoride (PMSF).

기타 정보

Stored properly at 2-8°C, in an unopened bottle, DIFP should be stable for a minimum of two years. DIFP
is unstable when exposed to moisture. DIFP will develop a dark yellow color upon decomposition.

안전성 정보

• 픽토그램: GHS06
• 신호어: Danger
• 유해 및 위험 성명서: H300 + H310 + H330
• 예방조치 성명서: P260 - P262 - P264 - P280 - P302 + P352 + P310 - P304 + P340 + P310
• Hazard Classifications: Acute Tox. 1 Oral - Acute Tox. 2 Dermal - Acute Tox. 2 Inhalation
• Storage Class Code: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter