T7009

Thrombin from human plasma

lyophilized powder, ≥1,000 NIH units/mg protein (E1%/280, 18.3)

• 동의어(들): Factor IIa
• CAS Number: 9002-04-4
• 효소 위원회 번호: 3.4.21.5 (BRENDA, IUBMB)
• EC Number: 232-648-7
• MDL number: MFCD00082072
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 형태: lyophilized powder
• Quality Level: 200
• 특이 활성도: ≥1,000 NIH units/mg protein (E1%/280, 18.3)
• 분자량: 37.4 kDa
• 불순물: HIV and HBsAg, source material tested negative
• UniProt 수납 번호: P00734
• 응용 분야: diagnostic assay manufacturing
• 저장 온도: −20°C
• 유전자 정보: human ... F2(2147)

설명

일반 설명

The F2 gene encodes thrombin protein and is mapped to human chromosome 11p11.2.

애플리케이션

Thrombin from human plasma has been:

• dissolved in CaC_l2 and added to poly ethylene glycolated fibrinogen/cell solution for fibrin production
• to polymerize fibrin on well plates
• used as an endogenous agonist proteinase for proteinase-activated receptors : PAR1, PAR3 and PAR4 for stimulation of cells

생화학적/생리학적 작용

Thrombin is an endolytic serine protease which stimulates vascular damage induced-leukocytes, platelets, endothelial and mesenchymal cell production. This action is mediated via G-protein-coupled receptor. It catalysis the cleavage of fibrinogen at Arg−Gly bonds to form fibrin and release fibrinopeptides A and B. The predominant form of thrombin in vivo is the zymogen prothrombin (factor II), which is produced in the liver. Prothrombin is a coagulation activation factor, high levels of which indicates cancer. Thrombin can be used to cleave fusion proteins. Cleavage of fusion proteins can be carried out at a thrombin:fusion protein ratio of 1:500.

Serine protease that selectively cleaves Arg-Gly bonds in fibrinogen to form fibrin and fibrinopeptides A and B.

단위 정의

Activity is expressed in NIH units obtained by direct comparison to a NIH Thrombin Reference Standard

물리적 형태

Lyophilized from saline sodium citrate buffer, pH 6.5

분석 메모

The NIH assay procedure uses 0.2 ml diluted plasma (1:1 with saline) as a substrate and 0.1ml of thrombin sample (stabilized in a 1% buffered albumin solution) based on a modification of the method of Biggs. Only clotting times in the range of 15-25 seconds are used for determining thrombin concentrations.

기타 정보

View more information on thrombin at www.sigma-aldrich.com/enzymeexplorer.

면책조항

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H334
• 예방조치 성명서: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable