AB3848-I
Anti-phospho Smad1/Smad5/Smad8 (Ser463/465)
from rabbit, purified by affinity chromatography
동의어(들):
Mothers against decapentaplegic homolog 1, BSP-1, hSMAD1, JV4-1, MAD homolog 1, Mothers against DPP homolog 1, Mad-related protein 1, SMAD 1, SMAD family member 1, Smad1, Transforming growth factor-beta-signaling protein 1
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모든 사진(3)
About This Item
UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.41
추천 제품
생물학적 소스
rabbit
Quality Level
항체 형태
affinity isolated antibody
항체 생산 유형
primary antibodies
클론
polyclonal
정제법
affinity chromatography
종 반응성
human, rat, mouse
종 반응성(상동성에 의해 예측)
Xenopus (based on 100% sequence homology), mink (based on 100% sequence homology)
기술
immunohistochemistry: suitable (paraffin)
western blot: suitable
NCBI 수납 번호
UniProt 수납 번호
배송 상태
ambient
타겟 번역 후 변형
phosphorylation (pSer463/pSer465)
유전자 정보
human ... SMAD1(4086)
일반 설명
Mothers against decapentaplegic homolog 1 (UniProt Q15797; also known as BSP-1, hSMAD1, JV4-1, MAD homolog 1, Mothers against DPP homolog 1, Mad-related protein 1, SMAD 1, SMAD family member 1, Smad1, Transforming growth factor-beta-signaling protein 1) is encoded by the SMAD1 (also known as BSP1, MADH1, MADR1) gene (Gene ID 4086) in human. Mammalian SMADs constitute a family of transcription factors that are subdivided into three groups. SMAD1, 2, 3, 5, and 9 (a.k.a. SMAD8) are receptor-regulated SMADs or R-SMADs. SMAD4 is the only known mammalian common mediator SMAD or Co-SMAD and it mediates both the TGF- and BMP signalling pathways. SMAD6 and SMAD7 are inhibitory SMADs or I-SMADs that block the activation of R-SMADs by Co-SMAD via competitive binding. SMADs share a similar structure feature consisting of the conserved N-terminal Mad-homology 1 (MH1; a.a. 12-136 of hSMAD1, a.a. 13-137 of hSMAD2, a.a. 16-140 of hSMAD8) and C-terminal MH2 (a.a. 271-465 of hSMAD1 & hSMAD2, a.a. 273-467 of hSMAD8) domains joined by a non-conserved linker region among SMADs. MH1 domain in R-SMADs and Co-SMADs contains N-terminus nuclear localization signals (NLS), while SMAD4 has nuclear export signals (NES) in this domain. The MH2 domain is responsible for gene transactivation as well as for mediating SMAD-receptor, SMAD-SMAD, SMAD-coactivators and SMAD-corepressors interactions. A TGF- ligand (TGF- for SMAD2/3 and BMP for SMAD1/5/8) initiates signalling by binding and engaging type I and type II receptor serine/threonine kinases on the cell surface, allowing T RII-dependent phosphorylation of T RI kinase domain, which then instigates signalling by phosphorylating the last two serine residues within the conserved C-terminal end Ser-Ser-X-Ser (SSXS) motif of SMAD proteins.
특이성
This rabbit polyclonal antibody recognizes SMAD1/5/8 phosphorylated on their last two serine residues within the C-terminal end Ser-Ser-X-Ser (SSXS) motif. Target serine residues are present in human/mouse SMAD1 (S463/S465), rat SMAD1 (S466/S468), human/mouse/rat SMAD5 (S463/S465), human SMAD9 (S465/S467), mouse SMAD9 (S428/S430), and rat SMAD9 (S432/S434), but absent in human SMAD1 spliced isoform 2. SMAD9 is also known as SMAD8. Target bands detection was blocked by the immunogen phosphopeptide, but not by the corresponding non-phosphopeptide.
면역원
Epitope: C-terminus
KLH-conjugated linear peptide corresponding to human SMAD1 C-terminal end sequence phosphorylated on the last two serine residues (S463/S465).
애플리케이션
Anti-phospho Smad1/Smad5/Smad8 (Ser463/465) Antibody, Cat. No. AB3848-I, is a highly specific rabbit polyclonal antibody that targets Smad1//5/8 phosphorylation and has been tested in Immunohistochemistry and Western Blotting.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Smad1/5/8 phosphorylation in mouse & rat embryo tissue sections.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
품질
Evaluated by Western Blotting in HEK293 cell lysates.
Western Blotting Analysis: A 1:500 dilution of this antibody detected an increased SMAD1/5/8 phosphorylation in 10 µg lysate from BMP2-treated HEK293 cells. Target bands detection was blocked by the immunogen phosphopeptide, but not the corresponding non-phosphopeptide.
Western Blotting Analysis: A 1:500 dilution of this antibody detected an increased SMAD1/5/8 phosphorylation in 10 µg lysate from BMP2-treated HEK293 cells. Target bands detection was blocked by the immunogen phosphopeptide, but not the corresponding non-phosphopeptide.
표적 설명
~55 kDa observed. 52.26/52.16/52.71 kDa (human/mouse/rat SMAD1), 52.26/52.17/52.22 kDa (human/mouse/rat SMAD5), and 52.49/48.64/48.99 kDa Uncharacterized bands may be observed in some lysate(s).
물리적 형태
Affinity purified.
Purified rabbit polyclonal antibdoy in buffer containing 0.1 M Tris-Glycine (pH 7.4) 150 mM NaCl with 0.05% sodium azide
저장 및 안정성
Stable for 1 year at 2-8°C from date of receipt.
기타 정보
Concentration: Please refer to lot specific datasheet.
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
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