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Merck
모든 사진(1)

주요 문서

BSAV-RO

Roche

Bovine Serum Albumin

≥98.5%, New Zealand origin

동의어(들):

Bovine Serum Albumin Fraction V

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About This Item

UNSPSC 코드:
12352202

생물학적 소스

bovine

Quality Level

분석

≥98.5%

양식

lyophilized

분자량

Mr =68 kDa

포장

pkg of 1 kg (10735108001)
pkg of 100 g (10735086001)
pkg of 50 g (10735078001)
pkg of 500 g (10735094001)

제조업체/상표

Roche

기원

New Zealand origin

기술

ELISA: suitable
Southern blotting: suitable
western blot: suitable

불순물

<0.0003 Lithium (Flame photometry)
<0.001 Iron (bathophenanthraline)
<0.002 P (inorganic)
<0.003 Heavy metals (as Pb)
<0.003 Magnesium (xylidyl blue)
<0.005 Glycerol (enzyme)
<0.006 potassium (Flame photometry)
<0.02 Calcium ( o-cresolphthalein)
<0.05 glucose (enzyme)
<0.1 L-lactate
<0.15 chloride (mercurom)
<0.5 Sodium (flame photometry)
<100 microorganisms (organism/g)
<5 water

흡수

≤0.4 at 550 nm

UniProt 수납 번호

외래 활성

Microorganisms <100 (Organism/g)

저장 온도

2-8°C

유전자 정보

bovine ... ALB(280717)

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일반 설명

For standard applications.

애플리케이션

Bovine Serum Albumin Fraction V is suitable for standard applications such as:
  • Stabilizer to prevent proteolysis and denaturation of enzymes and other proteins, especially when the proteins are present in low concentrations.
  • Buffer component in immunochemistry, biochemistry, cell biology, or molecular biology
  • Reducing agent
  • Protein blocker for saturation of protein binding sites in ELISAs, Southern blots, and western blots
  • Media supplement for media that require addition of protein
  • Carrier protein
  • Standard protein in gel electrophoresis and protein determination

품질

Purity: ≤95% protein, ≤5% H2O; electrophoretically homogeneous
Contaminants: <0.5% Na (flame photometric), <0.006% K (flame photometric), <0.0003% Li (flame photometric), <0.02% Ca (o-cresolphthalein), <0.003% Mg (xylidyl blue), <0.003% heavy metals (as Pb), <0.001% Fe (bathophenanthroline), <0.002% Pi, <0.05% glucose (enzymatic), <0.005% glycerol (enzymatic), <0.025% L-lactate (enzymatic)

제조 메모

Storage conditions (working solution): In solution, albumin is stable for about three months at -15 to -25 °C.

기타 정보

For life science research only. Not for use in diagnostic procedures.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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문서 라이브러리 방문

Francis K Y Siu et al.
Nature protocols, 3(1), 51-58 (2008-01-15)
Southwestern blotting is used to investigate DNA-protein interactions. The advantage of this technique over other related methods such as electrophoretic mobility shift assay (EMSA) and DNA footprinting is that it provides information regarding the molecular weight of unknown protein factor.
Laurent Berchebru et al.
Journal of microbiological methods, 105, 141-145 (2014-07-20)
Test and evaluation of engineered biothreat agent detection systems ("biodetectors") are a challenging task for government agencies and industries involved in biosecurity and biodefense programs. In addition to user friendly features, biodetectors need to perform both highly sensitive and specific
Paulina Krzysica et al.
Animals : an open access journal from MDPI, 12(21) (2022-11-12)
Cytokines like interferon (IFN)-γ, interleukin (IL)-2, IL-6, IL-10, and IL-12p40 are important biomarkers for characterizing the nature and strength of immune responses. It is important to be able to quantify the cytokines at the protein level in biological samples. Quantification
Leonie Kollenstart et al.
Scientific reports, 11(1), 12795-12795 (2021-06-19)
The collection of known posttranslational modifications (PTMs) has expanded rapidly with the identification of various non-acetyl histone lysine acylations, such as crotonylation, succinylation and butyrylation, yet their regulation is still not fully understood. Through an unbiased chromatin immunoprecipitation (ChIP)-based approach
Mariko Kuse et al.
The Journal of reproduction and development, 59(4), 346-352 (2013-04-09)
Cortisol (Cr), the most important glucocorticoid (GC), is well known to suppress uterine prostaglandin F2α (PGF) production. However, the details of the regulatory mechanisms controlling the cyclic changes in endometrial PGF production remain unclear. Here we investigated the expression of

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