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UHPLC (Isocratic) Analysis of 14 Cannabinoids on Ascentis® Express C18

UHPLC (Isocratic) Analysis of 14 Cannabinoids on Ascentis® Express C18 application for UHPLC

材料

付属品

製品番号
詳細
価格

51163-U

EXP®Pre-カラムフィルター

holder with EXP titanium hybrid ferrule, pack of 1 sets

51164-U

EXP® Pre-Column Filter Cartridges

0.5μm, pkg of 5 ea

分析カラム

製品番号
詳細
価格

53825-U

Ascentis® Express C18, 2.7 µm HPLCカラム

2.7 μm particle size, L × I.D. 15 cm × 2.1 mm

標準

製品番号
詳細
価格

C-045

カンナビジオール 溶液

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

C-046

カンナビノール 溶液

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

T-005

(−)-trans-Δ9-THC solution

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

T-032

(−)-Δ8-THC solution

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

C-140

カンナビジバリン 溶液

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

C-141

Cannabigerol 溶液

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

C-142

カンナビゲロール酸 溶液

1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant®

C-143

カンナビクロメン 溶液

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

C-144

カンナビジオール酸 溶液

1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant®

T-094

テトラヒドロカンナビバリン (THCV) 溶液

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

T-093

Delta9-Tetrahydrocannabinolic acid A 溶液

1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant®

C-152

カンナビジバリン酸（CBDVA） 溶液

1.0 mg/mL in acetonitrile, certified reference material, ampule of 1 mL, Cerilliant®

C-150

Cannabichromenic Acid (CBCA) solution

1.0 mg/mL in acetonitrile, certified reference material, ampule of 1 mL, Cerilliant®

CONDITIONS

column

Ascentis Express C18, 15 cm x 2.1 mm, 2.7 μm particles (53825-U)

mobile phase

[A] 0.1% formic acid in HPLC water; [B] 0.1% formic acid in acetonitrile; (30:70, A:B)

flow rate

0.6 mL/min

pressure

6390 psi (440 bar)

column temp.

20 °C

detector

UV, 230 nm

injection

2 μL

sample

25 μg/mL each compound in acetonitrile

詳細

アナリシスノート

Cannabinoids are a group of active compounds found in cannabis. They reportedly provide relief to an assortment of symptoms in the treatment of pain, nausea, anxiety, mood disorders, and inflammatory diseases. Cannabinoids imitate naturally occurring compounds and interact with two specific types of receptors: CB1 and CB2, located in the nervous system and immune system, respectively. Shown here is an isocratic separation of fourteen cannabinoids in less than twelve minutes on an Ascentis® Express C18 column. Cerilliant cannabinoid CRMs provide reliable identification and quantification. These standards are used in testing methods by GC/MS, LC/MS, or HPLC for applications in clinical toxicology, testing of cannabis potency or impurity profiling by growers, pharmaceutical research, forensic analysis, and urine drug testing.

法的情報

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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