LC/MS/MS Analysis of Vitamin D Metabolites on Ascentis® Express F5 (Monitored Ions)
材料
分析カラム
標準
25-ヒドロキシビタミンD2 溶液
50 μg/mL in ethanol, ampule of 1 mL, certified reference material, Cerilliant®25-ヒドロキシビタミンD3 溶液
100 μg/mL in ethanol, ampule of 1 mL, certified reference material, Cerilliant®1α,25-ジヒドロキシビタミン D3 溶液
5 μg/mL in ethanol, ampule of 1 mL, certified reference material, Cerilliant®1α,25-Dihydroxyvitamin D2 solution
5 μg/mL in ethanol, ampule of 1 mL, certified reference material, Cerilliant®移動相成分
CONDITIONS
column
Ascentis Express F5, 15 cm x 2.1 mm I.D., 2.7 μm particles (53571-U)
mobile phase
[A] 5 mM ammonium formate water; [B] methanol; (25:75, A:B)
flow rate
0.4 mL/min
pressure
5178 psi (357 bar)
column temp.
40 °C
detector
ESI(+), 100-1000 m/z
injection
2 μL, each compound 300 ng/mL in 40:60 (water:methanol)
詳細
アナリシスノート
The metabolic role and health implications of the various vitamin D isoforms are of current clinical interest. Therefore, it is important to have an analytical method that will resolve all of the known isoforms with necessary sensitivity and specificity.This application shows that a combination of Ascentis Express F5 with MS detection provided rapid resolution of seven such compounds. The highest grade LC-MS solvents were used to supply low background interference and low particulate contaminants for robust, trouble-free operation. Cerilliant CRMs provided reliable identification and quantification.
法的情報
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany