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HPLC Analysis of Sugars on Ascentis® Express HILIC (Mobile Phase 5:95)

HPLC Analysis of Sugars on Ascentis® Express HILIC (Mobile Phase 5:95) application for HPLC

材料

Standard

製品番号
詳細
価格

D-(+)-セロビオース

≥98% (HPLC)

L-(+)-アラビノース

≥99% (GC)

スクロース

BioUltra, for molecular biology, ≥99.5% (HPLC)

Galactose

United States Pharmacopeia (USP) Reference Standard

グルコース

European Pharmacopoeia (EP) Reference Standard

D-(+)-キシロース

European Pharmacopoeia (EP) Reference Standard

D-(+)-マンノース

United States Pharmacopeia (USP) Reference Standard

分析カラム

製品番号
詳細
価格

Ascentis® Express HILIC、2.7 μm HPLCカラム

2.7 μm particle size, L × I.D. 15 cm × 4.6 mm

CONDITIONS

column

Ascentis Express HILlC, 15 cm x 4.6 mm I.D., 2.7 μm particles (53981-U)

mobile phase

[A] water; [B] acetonitrile; (5:95, A:B)

flow rate

1.0 mL/min

column temp.

35 °C

detector

ELSD, 55 °C, 3.5 bar nitrogen

injection

10 μL

詳細

アナリシスノート

This application demonstrates the suitability of the Ascentis Express HILIC for the analysis of sugars. Structures along with the optimized chromatogram obtained on the Ascentis Express HILIC are presented. Separation is by normal-phase mechanism as a decrease in mobile phase water content results in increased retention

法的情報

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany