HPLC Analysis of Sugars on Ascentis® Express HILIC (Mobile Phase 5:95)
材料
Standard
製品番号
詳細
価格
分析カラム
製品番号
詳細
価格
CONDITIONS
column
Ascentis Express HILlC, 15 cm x 4.6 mm I.D., 2.7 μm particles (53981-U)
mobile phase
[A] water; [B] acetonitrile; (5:95, A:B)
flow rate
1.0 mL/min
column temp.
35 °C
detector
ELSD, 55 °C, 3.5 bar nitrogen
injection
10 μL
詳細
アナリシスノート
This application demonstrates the suitability of the Ascentis Express HILIC for the analysis of sugars. Structures along with the optimized chromatogram obtained on the Ascentis Express HILIC are presented. Separation is by normal-phase mechanism as a decrease in mobile phase water content results in increased retention
法的情報
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany