HPLC Analysis of Fat Soluble Vitamins by Normal Phase Chromatography on Ascentis® Si (25 cm Column)
CONDITIONS
column
Ascentis Si, 15 cm x 4.6 mm I.D., 5 μm particles (581512-U)
mobile phase
[A] hexane; [B] ethyl acetate
gradient
10 to 30% B in 10 min; held at 30% B for 2 min
flow rate
1.0 mL/min
column temp.
30 °C
detector
UV, 290 nm
injection
10 μL
詳細
アナリシスノート
Ascentis silica is used in the analysis of fat-soluble vitamins using a normal phase gradient. These include vitamin K 3 (menadione), vitamin E (alpha and gamma tocopherol), and vitamin D3 (cholecalciferol).
法的情報
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany