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HPLC Analysis of Drugs of Abuse on Ascentis® Express F5

HPLC Analysis of Drugs of Abuse on Ascentis® Express F5 application for HPLC

材料

Standard

製品番号
詳細
価格

Codeine monohydrate

analytical standard

モルヒネ 硫酸塩 五水和物

analytical standard

Benzoylecgonine solution

100 μg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

Fentanyl solution

100 μg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

ヒドロコドン 溶液

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

(±)-MDMA solution

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

(±)-Methamphetamine solution

100 μg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

Meperidine solution

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

Normeperidine solution

100 μg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

(+)-Propoxyphene solution

1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant®

分析カラム

製品番号
詳細
価格

Ascentis® Express HILIC、2.7 μm HPLCカラム

2.7 μm particle size, L × I.D. 7.5 cm × 3 mm

CONDITIONS

column

Ascentis Express F5, 10 cm x 2.1 mm I.D., 2.7 μm particles (53569-U)

mobile phase

[A] 5 mM ammonium formate; [B] 5 mM ammonium formate in 95:5 (v/v) acetonitrile:water

gradient

held at 20% B for 0.5 min; 20 to 100% B in 5.5 min; held at 100% B for 4 min

flow rate

0.3 mL/min

pressure

175 bar

column temp.

35 °C

detector

ESI(+), m/z 100-1000

injection

1 μL

sample

1 μg/mL in 50:50, water: methanol

詳細

法的情報

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany