HPLC Analysis of Caffeine and Taurine on Ascentis® Express HILIC Column using Evaporative Light Scattering Detection (ELSD, Fast Analysis)
CONDITIONS
column
Ascentis Express HILIC, 5 cm x 3.0 mm I.D., 2.7 μm particles (53967-U)
mobile phase
[A] 100 mM ammoniumformate, pH 3.0 with formic acid; [B] acetonitrile; (15:85, A:B)
flow rate
0.6 mL/min
column temp.
25 °C
detector
ELSD, 55 °C, 3.5 bar nitrogen
injection
2 μL
sample
1000 μg/mL in 10:90, water: acetonitrile
詳細
アナリシスノート
This application demonstrates the suitability of Ascentis Express HILIC for the efficient separation of caffeine and taurine. Elution is by aqueous normal phase (ANP) as an increase in organic will increase retention.
法的情報
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany