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HPLC Analysis of an Energy Drink on Ascentis® Express HILIC (UV Detection)

HPLC Analysis of an Energy Drink on Ascentis® Express HILIC (UV Detection) application for HPLC

材料

分析カラム

製品番号
詳細
価格

Ascentis® Express HILIC、2.7 μm HPLCカラム

2.7 μm particle size, L × I.D. 10 cm × 3 mm

標準

製品番号
詳細
価格

カフェイン

Sigma Reference Standard, vial of 250 mg

ソルビン酸

99.0-101.0% anhydrous basis

ピリドキシン塩酸塩(B6)

analytical standard

Nicotinamide (Niacinamide)

analytical standard

リボフラビン (B2)

analytical standard

安息香酸

2000 μg/mL in dichloromethane, analytical standard

CONDITIONS

column

Ascentis Express HILlC, 10 cm x 3.0 mm I.D., 2.7 μm particles (53970-U)

mobile phase

[A] 100 mM ammonium acetate, pH 5.0 with acetic acid; [B] water; [C] acetonitrile; (09:01:90, A:B:C)

flow rate

0.6 mL/min

pressure

812 psi (56 bar)

column temp.

35 °C

detector

UV, 254 nm

injection

2 μL

sample

Dilute 1:9 in acetonitrile

詳細

アナリシスノート

This application demonstrates the suitability of Ascentis Express HILIC for the efficient separation of common drink additives used in caffeinated energy drinks. The analysis was performed with UVand ELSD detectors in series, this chromatogram being from the UV.

法的情報

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany