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HPLC Analysis of Aminopterin and Related Compounds on Ascentis® Express C18

HPLC Analysis of Aminopterin and Related Compounds on Ascentis® Express C18 application for HPLC

材料

Standard

製品番号
詳細
価格

F7876

葉酸

≥97%

M9929

メトトレキサート

meets USP testing specifications

分析カラム

製品番号
詳細
価格

53829-U

Ascentis® Express C18, 2.7 µm HPLCカラム

2.7 μm particle size, L × I.D. 15 cm × 4.6 mm

CONDITIONS

column

Ascentis Express C18, 15 cm x 4.6 mm I.D., 2.7 μm particles (53829-U)

mobile phase

[A] 10 mM ammonium formate, pH 3.0; [B] acetonitrile; (90:10, A:B)

flow rate

1.0 mL/min

column temp.

35 °C

detector

UV, 280 nm

injection

5 μL

詳細

アナリシスノート

This application demonstrates the suitability of the Ascentis C18 for the analysis of aminopterin, folic acid and methotrexate. Structures along with the optimized chromatogram obtained on the Ascentis Express C18 are presented

法的情報

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Chromatogram Search

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