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Merck

Long-Term Expansion of Pancreatic Islet Organoids from Resident Procr+ Progenitors.

Cell (2020-03-24)
Daisong Wang, Jingqiang Wang, Lanyue Bai, Hong Pan, Hua Feng, Hans Clevers, Yi Arial Zeng
要旨

It has generally proven challenging to produce functional β cells in vitro. Here, we describe a previously unidentified protein C receptor positive (Procr+) cell population in adult mouse pancreas through single-cell RNA sequencing (scRNA-seq). The cells reside in islets, do not express differentiation markers, and feature epithelial-to-mesenchymal transition characteristics. By genetic lineage tracing, Procr+ islet cells undergo clonal expansion and generate all four endocrine cell types during adult homeostasis. Sorted Procr+ cells, representing ∼1% of islet cells, can robustly form islet-like organoids when cultured at clonal density. Exponential expansion can be maintained over long periods by serial passaging, while differentiation can be induced at any time point in culture. β cells dominate in differentiated islet organoids, while α, δ, and PP cells occur at lower frequencies. The organoids are glucose-responsive and insulin-secreting. Upon transplantation in diabetic mice, these organoids reverse disease. These findings demonstrate that the adult mouse pancreatic islet contains a population of Procr+ endocrine progenitors.

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製品内容

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HEPES, BioPerformance Certified, ≥99.5% (titration), suitable for cell culture
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ホルムアルデヒド 溶液, for molecular biology, 36.5-38% in H2O
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Triton X-100, laboratory grade
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タージトール 溶液, Type NP-40, 70% in H2O
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ノコダゾール, ≥99% (TLC), powder
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グルタルアルデヒド 溶液, Grade II, 25% in H2O
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四酸化オスミウム 溶液, suitable for electron microscopy, 4% in H2O
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パラホルムアルデヒド, reagent grade, crystalline
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ヘキサシアノ鉄(II)酸カリウム 三水和物, ≥99.95% trace metals basis
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モノクロナール抗グルカゴン マウス宿主抗体, clone K79bB10, ascites fluid
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ヘパリン ナトリウム塩 from porcine intestinal mucosa, ≥180 USP units/mg
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モノクロナール抗インスリン マウス宿主抗体, clone K36AC10, ascites fluid
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クエン酸鉛(II) 三塩基性 三水和物, purum, suitable for electron microscopy
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Anti-SST antibody produced in rabbit, affinity isolated antibody, buffered aqueous glycerol solution
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抗マウスIgG (全分子)–金 ヤギ宿主抗体, affinity isolated antibody, aqueous glycerol suspension, 10 nm (colloidal gold)
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抗膵臓ポリペプチド/PPY ヤギ宿主抗体, affinity isolated antibody, buffered aqueous solution